Protein Info for GFF1498 in Methylophilus sp. DMC18

Annotation: Protease HtpX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 289 transmembrane" amino acids 9 to 26 (18 residues), see Phobius details amino acids 32 to 49 (18 residues), see Phobius details amino acids 149 to 171 (23 residues), see Phobius details amino acids 184 to 204 (21 residues), see Phobius details PF01435: Peptidase_M48" amino acids 79 to 284 (206 residues), 130.1 bits, see alignment E=4.5e-42

Best Hits

KEGG orthology group: K03799, heat shock protein HtpX [EC: 3.4.24.-] (inferred from 76% identity to meh:M301_0212)

Predicted SEED Role

"Peptidase M48, Ste24p precursor"

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.-

Use Curated BLAST to search for 3.4.24.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (289 amino acids)

>GFF1498 Protease HtpX (Methylophilus sp. DMC18)
MLGNWTKTFVLMAAITALFVAVGGALGGQGGMLLALLLAGGMNFYAYWFSDQAVLKMYGA
VEINAENNFGNGQFRNYYNMVKELAERAQLPMPRVFVINESQPNAFATGRNPENAAVAAT
TGIMQILSERELRGVMAHELAHVKHRDTLISTISATVAGAISSIAQFGLMFGGHREGEDR
PNPIIGILIMILAPLAASLIQMAISRSREYEADRMGAEISRDPKALAAALEKIHNYAHQI
PNMTAEQHPETGQMMIINPLSGADLAGLFSTHPQTEERIRRLLALANQY