Protein Info for GFF1497 in Methylophilus sp. DMC18

Annotation: Methionyl-tRNA formyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 306 TIGR00460: methionyl-tRNA formyltransferase" amino acids 1 to 303 (303 residues), 305.5 bits, see alignment E=2e-95 PF00551: Formyl_trans_N" amino acids 1 to 179 (179 residues), 154 bits, see alignment E=3.7e-49 PF02911: Formyl_trans_C" amino acids 204 to 297 (94 residues), 84.2 bits, see alignment E=6.2e-28

Best Hits

Swiss-Prot: 61% identical to FMT_METFK: Methionyl-tRNA formyltransferase (fmt) from Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)

KEGG orthology group: K00604, methionyl-tRNA formyltransferase [EC: 2.1.2.9] (inferred from 66% identity to mep:MPQ_0196)

MetaCyc: 52% identical to 10-formyltetrahydrofolate:L-methionyl-tRNAfMet N-formyltransferase (Escherichia coli K-12 substr. MG1655)
Methionyl-tRNA formyltransferase. [EC: 2.1.2.9]; 2.1.2.9 [EC: 2.1.2.9]

Predicted SEED Role

"Methionyl-tRNA formyltransferase (EC 2.1.2.9)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or Folate Biosynthesis (EC 2.1.2.9)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (306 amino acids)

>GFF1497 Methionyl-tRNA formyltransferase (Methylophilus sp. DMC18)
MRIIYAGTPEFAVPALQGLIDAGHDIVMVLTQPDRPAGRGMQLKASPVKQLALQHGLRVF
QPESLKPAEVQSEIAAVNAEVLIVAAYGLIIPTAVLNIPTRGCYNIHGSLLPRWRGAAPI
HRAILAGDAETGVTIMEVVPKLDAGNMISKWSVAITAADTTQTLHDAISREGARLMVEAM
QTLQATGRLASEVQDEALVTYAHKLEKAESAIDWTQSAQQLSRQVRAFNPFPVATAKFND
QVCKLWFAQAVAGQGEPGTVLQTHPLQVACGDGALELHELQMPGGKRQTAQQFVQGQHVQ
VGDRFF