Protein Info for GFF1487 in Pseudomonas sp. DMC3

Annotation: Multidrug export protein EmrA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 transmembrane" amino acids 24 to 43 (20 residues), see Phobius details PF00529: CusB_dom_1" amino acids 36 to 347 (312 residues), 35 bits, see alignment E=2.7e-12 PF13533: Biotin_lipoyl_2" amino acids 63 to 111 (49 residues), 25.7 bits, see alignment 2e-09 PF16576: HlyD_D23" amino acids 166 to 293 (128 residues), 50.6 bits, see alignment E=4e-17 PF13437: HlyD_3" amino acids 219 to 299 (81 residues), 54.4 bits, see alignment E=4.9e-18

Best Hits

Swiss-Prot: 47% identical to EMRA_ECOLI: Multidrug export protein EmrA (emrA) from Escherichia coli (strain K12)

KEGG orthology group: K03543, multidrug resistance protein A (inferred from 95% identity to pfl:PFL_3917)

MetaCyc: 47% identical to multidrug efflux pump membrane fusion protein EmrA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-363; TRANS-RXN-364; TRANS-RXN-365

Predicted SEED Role

"Membrane fusion component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (400 amino acids)

>GFF1487 Multidrug export protein EmrA (Pseudomonas sp. DMC3)
MATAENTQAQDNTPDTGNSRKRKVMLLALAVVVLLAIAGVWAYHEFIGRFNESTDDAYVN
GNVVEITPLVTGTVVSIGADDGDLVQEGQVLVNFDPNDAEVGLQSAKAKLARTVRQVRGL
YSNVDGMKAQVNAQQAEVQKAQDNYNRRKNLAQGGAISQEELSHARDDLTSAQNALANAK
QQLKTTSALVDDTVVSSHPDVMSAAADLRQAYLTNARSTLIAPVTGYVAKRTVQLGQRVQ
PGTALMAVIPLDQLWIDANFKETQLRDMRIGQPVDIEADIYGSDVKYSGTVDSLGAGTGS
AFALLPAQNATGNWIKIVQRVPVRIHINAEELAKHPLRVGLSTNVEVNLHDQSGPVLAQQ
PPQKASFSTNVYERQLAEADAMIAQLIHDNSAAAGKTAQR