Protein Info for GFF1480 in Variovorax sp. SCN45

Annotation: Choline-sulfatase (EC 3.1.6.6)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 507 TIGR03417: choline-sulfatase" amino acids 2 to 487 (486 residues), 592.2 bits, see alignment E=4.9e-182 PF00884: Sulfatase" amino acids 4 to 345 (342 residues), 164.9 bits, see alignment E=4.2e-52 PF01663: Phosphodiest" amino acids 7 to 292 (286 residues), 40.9 bits, see alignment E=3e-14 PF16347: SGSH_C" amino acids 296 to 435 (140 residues), 46 bits, see alignment E=9.7e-16

Best Hits

KEGG orthology group: K01133, choline-sulfatase [EC: 3.1.6.6] (inferred from 52% identity to hel:HELO_1581)

Predicted SEED Role

"Choline-sulfatase (EC 3.1.6.6)" in subsystem Choline Transport or Choline and Betaine Uptake and Betaine Biosynthesis (EC 3.1.6.6)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.1.6.6

Use Curated BLAST to search for 3.1.6.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (507 amino acids)

>GFF1480 Choline-sulfatase (EC 3.1.6.6) (Variovorax sp. SCN45)
MKKPNILLIMADQLSAPVLPFHGNTAVKTPHLQALAEQSVVFDNAYCNFPLCAPARFSLL
AGQFATEIGAFDNACEFEASTPTLPYYLGREGYKTILSGKMHFVGPDQKHGFQERLTTDI
YPADFGWSADWKEGEFSFYSPGHNLSTVDESGECFRSLQLDYDEEVETKSIQRLYDLARD
DEQPFFMCVSYTHPHPPFHILPEYLARYDRAEINLPNVRDIPMKERDMLSQWVQYSHGLD
KQTATDEQILRARHAYYAMVSYIDDKVGNLLKTLERTGFDKNTIVVFTSDHGDMLGERGM
WFKRVFFDWSAKVPLLFKPPGATTPRRISEPASHVDLLPTLMAFATGKESSEWVEPVDGR
SLRPVIEGAAEHGDATAFVEYTAEGVTGPCCMLRKGHYKYVYTHGYPDQLYDLEADPDEL
RNVAAKLPDVLAALKAEVHTRWDADDIKSRVLHSQARRKFIKDLPDAIQPVWDYQASVDD
TKRYVRRGSARIIKVKKRWPVVADIKS