Protein Info for PS417_07505 in Pseudomonas simiae WCS417

Annotation: cysteine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 324 transmembrane" amino acids 269 to 283 (15 residues), see Phobius details TIGR01136: cysteine synthase" amino acids 10 to 313 (304 residues), 424.1 bits, see alignment E=3.2e-131 TIGR01139: cysteine synthase A" amino acids 10 to 313 (304 residues), 445.2 bits, see alignment E=1.1e-137 PF00291: PALP" amino acids 11 to 302 (292 residues), 246.4 bits, see alignment E=2e-77

Best Hits

Swiss-Prot: 71% identical to CYSK_ECOLI: Cysteine synthase A (cysK) from Escherichia coli (strain K12)

KEGG orthology group: K01738, cysteine synthase A [EC: 2.5.1.47] (inferred from 98% identity to pfs:PFLU1540)

MetaCyc: 71% identical to cysteine synthase A (Escherichia coli K-12 substr. MG1655)
Cystathionine gamma-lyase. [EC: 4.4.1.1, 4.4.1.28]; Cysteine synthase. [EC: 4.4.1.1, 4.4.1.28, 2.5.1.47]

Predicted SEED Role

"Cysteine synthase (EC 2.5.1.47)" in subsystem Cysteine Biosynthesis or Methionine Biosynthesis (EC 2.5.1.47)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.47

Use Curated BLAST to search for 2.5.1.47 or 4.4.1.1 or 4.4.1.28

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U6I6 at UniProt or InterPro

Protein Sequence (324 amino acids)

>PS417_07505 cysteine synthase (Pseudomonas simiae WCS417)
MSRIFADNAHSIGNTPLVQINRIAPRGVTILAKIEGRNPGYSVKCRIGANMIWDAESTGK
LKPGMTIVEPTSGNTGIGLAFVAAARGYKLLLTMPASMSIERRKVLKALGAELVLTEPAK
GMKGAIEKAGEIVASDPATYFMPAQFENPANPAIHEKTTGPEIWNDTDGAVDVLVAGVGT
GGTITGVSRYIKNIAGKPILSVAVEPIVSPVITQALAGEEIKPSPHKIQGIGAGFVPKNL
DLSMVDRVELVTDDESKAMALRLMQEEGILCGISCGAAMAVAVRLAEKPEMQGKTIVVIL
PDSGERYLSSMLFSDLFTEQENQA