Protein Info for GFF147 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01082: UDP-N-acetylmuramate--L-alanine ligase
  • amino acids 21 to 474 (454 residues), 603.6 bits, E=1.3e-185
• PF01225: Mur_ligase
  • amino acids 21 to 119 (99 residues), 116.4 bits, E=9.3e-38
• PF08245: Mur_ligase_M
  • amino acids 124 to 304 (181 residues), 96.2 bits, E=4.1e-31
• PF02875: Mur_ligase_C
  • amino acids 337 to 466 (130 residues), 58.5 bits, E=2e-19

Best Hits

Swiss-Prot: 100% identical to MURC_SALSV: UDP-N-acetylmuramate--L-alanine ligase (murC) from Salmonella schwarzengrund (strain CVM19633)

KEGG orthology group: K01924, UDP-N-acetylmuramate--alanine ligase [EC: 6.3.2.8] (inferred from 99% identity to sec:SC0126)

MetaCyc: 95% identical to UDP-N-acetylmuramate--L-alanine ligase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylmuramate--L-alanine ligase. [EC: 6.3.2.8]

Predicted SEED Role

"UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.8)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (12/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• D-Glutamine and D-glutamate metabolism
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.8

Protein Sequence (491 amino acids)

>GFF147 UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGYQISGSDLAPNPVTQQL
TSLGATIFFNHRPENVRDASVVVVSSAISADNPEIVAAHEARIPVIRRAEMLAELMRFRH
GIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAGVHARLGHSRYLIAEADESDA
SFLHLQPMVAIVTNIEADHMDTYHGDFENLKQTFINFLHNLPFYGRAVMCVDDPVIRELL
PRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLRQGMPDLHVTLNAPGRHNALNAAAA
VAVATEEGIADDAILRALESFQGTGRRFDFLGEFPLEPVNGKAGTAMLVDDYGHHPTEVD
ATIKAARAGWPDKNLVMLFQPHRYTRTRDLYDDFANVLTQVDALLMLDVYPAGEAPIPGA
DSRSLCRTIRNRGKIDPILVSDPAQVATMLAPVLTGNDLILVQGAGNVGKIARYLSEIKL
KPQIQEEEQHG