Protein Info for GFF1455 in Xanthobacter sp. DMC5

Annotation: 1,4-alpha-glucan branching enzyme GlgB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 739 PF22019: GlgB_N" amino acids 20 to 106 (87 residues), 47.2 bits, see alignment E=3.9e-16 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 116 to 732 (617 residues), 919.5 bits, see alignment E=5.2e-281 PF02922: CBM_48" amino acids 139 to 215 (77 residues), 78.1 bits, see alignment E=1.1e-25 PF00128: Alpha-amylase" amino acids 278 to 355 (78 residues), 33.6 bits, see alignment E=5.7e-12 PF02806: Alpha-amylase_C" amino acids 638 to 735 (98 residues), 89.5 bits, see alignment E=3e-29

Best Hits

Swiss-Prot: 66% identical to GLGB1_XANC5: 1,4-alpha-glucan branching enzyme GlgB 1 (glgB1) from Xanthomonas campestris pv. vesicatoria (strain 85-10)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 66% identity to xcv:XCV0141)

MetaCyc: 56% identical to 1,4-alpha-glucan branching enzyme (Mycobacterium tuberculosis H37Rv)
1,4-alpha-glucan branching enzyme. [EC: 2.4.1.18]

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (739 amino acids)

>GFF1455 1,4-alpha-glucan branching enzyme GlgB (Xanthobacter sp. DMC5)
MNSVQMNRMEMSRVPPLDPAVLDQLASGRLDDPFAVLGPHEGPQGRYVRVFVPGAEAVAV
EPEAGPQALFLEPGPIDGLFCGPLPEGAYGLSIRWPKRTEETEDPYAFGPLLGDLDLHLF
SEGRHFRLTDALGAALVTVEGTAGTRFAVWAPNARRVSVVGDFNGWDGRRHAMRRRGTSG
VFEIFIPRVGPGALYKYEIVGPDGALLPLKADPVARATQLPPETASVVPSPRTHRWRDEA
FMASRAARNAPSAPISIYEVHAGSWLRDPATGQPLSWLGLAERLVPYVKDLGFTHVELLP
VAEHPFTGSWGYQPLGLFAPSRRFGEPEDFAAFVDACHRADIGVIIDFVPAHFPSDAHGL
ARFDGTALYEHADPREGFHQDWNTLIYNLGRREVAGFLIASALHWLEHFHVDGLRVDAVA
SMLYRDYSRKPGEWIPNRFGGRENLESVDFLRHLNAVVAERCPGAITLAEESTAWPGVSS
PSADGGLGFSYKWNMGWMHDTLHYMAREPVYRRHHHDELTFSLVYAFSERFVLPLSHDEV
VHGKGSLIGKMPGDTWQKRANLRALLGFMWTHPGKKLLFMGGEIGQEREWSHDREIDWFL
LDDPAHRGIQSLVRDLNRLYRELPALHALDDRPEGFRWIVGDDRENSVLAFLRLAPGAAP
VLAVINLTPVPRAGYRIGVPAAGRWREVLNSDAPLYGGSGMGNYGGVETREEPAHGEGQS
LDLTLPPLSTLLFVHTGEN