Protein Info for GFF1440 in Variovorax sp. SCN45

Annotation: Multidomain signal transduction protein including CheB-like methylesterase, CheR-like methyltransferase and BaeS-like histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 647 TIGR00229: PAS domain S-box protein" amino acids 29 to 150 (122 residues), 66 bits, see alignment E=1.7e-22 amino acids 153 to 275 (123 residues), 67.4 bits, see alignment E=6.6e-23 PF00989: PAS" amino acids 30 to 141 (112 residues), 42.5 bits, see alignment E=2.3e-14 amino acids 158 to 269 (112 residues), 39.4 bits, see alignment E=2.3e-13 PF13426: PAS_9" amino acids 42 to 144 (103 residues), 48.8 bits, see alignment E=3.1e-16 amino acids 170 to 272 (103 residues), 49 bits, see alignment E=2.6e-16 PF08448: PAS_4" amino acids 42 to 146 (105 residues), 35.3 bits, see alignment E=4.8e-12 amino acids 170 to 275 (106 residues), 35.5 bits, see alignment E=4.2e-12 PF00512: HisKA" amino acids 287 to 353 (67 residues), 47.5 bits, see alignment E=6.1e-16 PF02518: HATPase_c" amino acids 399 to 511 (113 residues), 87.7 bits, see alignment E=2.9e-28 PF00072: Response_reg" amino acids 530 to 637 (108 residues), 71.3 bits, see alignment E=3.1e-23

Best Hits

KEGG orthology group: None (inferred from 79% identity to vpe:Varpa_3015)

Predicted SEED Role

"Chemotaxis protein methyltransferase CheR (EC 2.1.1.80)" in subsystem Bacterial Chemotaxis (EC 2.1.1.80)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.80

Use Curated BLAST to search for 2.1.1.80

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (647 amino acids)

>GFF1440 Multidomain signal transduction protein including CheB-like methylesterase, CheR-like methyltransferase and BaeS-like histidine kinase (Variovorax sp. SCN45)
MAAGTPPATPDEAAQDLSRPLNLGLVTDADYRLMIDSITDYAIFFLDPSGIVLSWNTGAR
RLKGYEAHEVIGGPLSMFYPPELLEKNWPQHELEVAARVGQFQDEGWRLRKDGTRFWASV
VITRLMGPDGKLRGFSKITRDLTERQKNNELLKASEERFRLIVEGVKDYAIFMLDPSGYI
VSWNAGAKATKGYEAHEIIGKHFSVFYPPEVVASGFPEKELRVALEVGRYEDEGWRVRKD
GSRFWASVVITSLVDASGRHRGFAKVTRDLTERRRISTLEDEGRRITTFLAMLGHELRNP
LTPISNAVAIMERLGPGVDAKTLARMNSIIARQLRQITRLVDDLLDVGRITSGKIHIESK
PVKLSEVIDEALEMVRPSADAKHHAVVLRAPAEELWIAGDRARMIQVVCNLLNNAVKFTP
DGGRIEIWLRSDGRKAEIGVSDNGPGIAPEHLPRIFMLFAQGDQEISRPQGGLGLGLTLV
LQLVTLHGGEVNAYSKGIPGAGAEFIVRLPSVAAPLERRKDKGGEAGKMVLVVDDNRDAA
ETMCLLVESLGYPTRTAFDGPSALEAIKVEQPELVLMDIGLPGFSGVEVARRVRREVEQP
PVLIAVTGFGQVSDRELSLEAGFYAHLTKPIDVSQLEGLLERLLGKA