Protein Info for GFF1425 in Methylophilus sp. DMC18

Annotation: Adaptive-response sensory-kinase SasA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details amino acids 182 to 205 (24 residues), see Phobius details PF00512: HisKA" amino acids 227 to 289 (63 residues), 37.9 bits, see alignment E=1.5e-13 PF02518: HATPase_c" amino acids 342 to 445 (104 residues), 80.2 bits, see alignment E=1.6e-26

Best Hits

KEGG orthology group: None (inferred from 54% identity to del:DelCs14_4632)

Predicted SEED Role

"Sensor protein of zinc sigma-54-dependent two-component system" in subsystem Zinc resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (448 amino acids)

>GFF1425 Adaptive-response sensory-kinase SasA (Methylophilus sp. DMC18)
MQTHTHKVRRDLLLLWAVIAAVSALLVWLLIELSRQGAASQIAEARLHAAASCQAMQHGV
HLARLESASAQAGWQAVIDLALREEPGSEGGFWRPGQGVFAYAFPTYDGTGVKRDPPVAE
LEKITATAELASRSHQLILEVRPGLREAIVFAACPLTQAPGTSVAWVMKRVLLIQAEVLN
HLSLALALLIGLVVMSGAWLAWMLLRWQRQMATLMQQLAQSERMATLGRVSAGLAHEIRN
PLATMRMKVENAMAAAPERYQQRSQSAFEAVLAQTSRLESLISSLLALTHPLQIHKQSVD
LRAWLTSLTSLHQEAMTAQQARWKLSLSDAVVTRAQAQAVLDPDKMAQVMDNLILNALAH
IPVGGEIELGLDSVTSGHLRLWVADNGSGVPAHLRPQVFEPFSTARSGGTGLGLALAREI
VQAHGGELRLADTAAGARFEIELPWHTS