Protein Info for GFF1420 in Variovorax sp. SCN45

Annotation: Indolepyruvate oxidoreductase subunit IorB II (EC 1.2.7.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 506 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details transmembrane" amino acids 368 to 381 (14 residues), see Phobius details PF01558: POR" amino acids 13 to 204 (192 residues), 55.8 bits, see alignment E=6.9e-19 PF20169: DUF6537" amino acids 235 to 446 (212 residues), 135.1 bits, see alignment E=3e-43

Best Hits

Predicted SEED Role

"Indolepyruvate oxidoreductase subunit IorB II (EC 1.2.7.8)" in subsystem Aromatic amino acid interconversions with aryl acids (EC 1.2.7.8)

Isozymes

Compare fitness of predicted isozymes for: 1.2.7.8

Use Curated BLAST to search for 1.2.7.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (506 amino acids)

>GFF1420 Indolepyruvate oxidoreductase subunit IorB II (EC 1.2.7.8) (Variovorax sp. SCN45)
MTRSVCVLIAAMGGEGGGVLADWLIDAATRGGFPVQSTSVPGVAQRTGATTYYVEIYPVP
RAELGGREPVLSLTPTPGGLDLVAASELVEAGRAIQAGYVSRERTVLCASTHREFAVSEK
SAMSDGRYDSARVLQAAHAMAAHSALFDMRMLAQKNGTVINTVLFGAMARSGRLPFDRAA
CEEAIRRSGKAVEASLRGFAAGYEWTPGADAPEPARHPGRLPTSARLDALPEAIRSIVAA
GVAHTADFQDARYAGLFLDRVERVLQLERAVGDPRFPVSRETARHLALWMAFEDVIRVAD
LKSRRERIEQVRREVGAKAGEPVQLTEFLKPGLDEICSVLPPRMAAWARKRLAARAHVLQ
KGIHLRSHTLHGFMTLVALRSLRPLRRRMSRYVAEQQAIERWLRAVQDSLGRSPELAQEV
ALAGNLVKGYGETSERGHRNLAAILDDVERANAASPPDAGAVERIHKARKAALDDPEGRA
LAGALGLPPPQPVAQPIRFVRRRPAG