Protein Info for GFF1406 in Sphingobium sp. HT1-2

Annotation: Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.2.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 681 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details TIGR03075: PQQ-dependent dehydrogenase, methanol/ethanol family" amino acids 32 to 544 (513 residues), 489 bits, see alignment E=8.8e-151 PF13360: PQQ_2" amino acids 92 to 229 (138 residues), 61.4 bits, see alignment E=2.1e-20 amino acids 462 to 528 (67 residues), 26.8 bits, see alignment E=7.8e-10 PF01011: PQQ" amino acids 93 to 111 (19 residues), 21.3 bits, see alignment (E = 3.5e-08) amino acids 136 to 166 (31 residues), 27.2 bits, see alignment (E = 4.6e-10) amino acids 493 to 528 (36 residues), 25.6 bits, see alignment 1.6e-09 PF13570: PQQ_3" amino acids 116 to 154 (39 residues), 23 bits, see alignment 1.7e-08 amino acids 155 to 206 (52 residues), 19.4 bits, see alignment 2.3e-07 amino acids 471 to 510 (40 residues), 20.8 bits, see alignment 8.4e-08 PF13442: Cytochrome_CBB3" amino acids 596 to 669 (74 residues), 41 bits, see alignment E=4e-14

Best Hits

KEGG orthology group: None (inferred from 84% identity to sch:Sphch_0592)

Predicted SEED Role

"Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8)" (EC 1.1.99.8)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.8

Use Curated BLAST to search for 1.1.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (681 amino acids)

>GFF1406 Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.2.8) (Sphingobium sp. HT1-2)
MKGRVSTAVAILALASGLVACGADSAAPGGPTANWSSFGGDDKEQHYSALDQISADNVGK
LGLAWSYDIDTYDSYTQPLAIDGVLYFAVGLSVVHALDAKTGKLLWQYDPDVASAPEAKW
RMRAGWGTRGIAYKDGLIYTATREGRLIAVDAKTGKPRWSVQTLDEAENGYITGPPWVAG
DKVVVGFGGADYSPTRGYVTAYDAKTGKKAWRWFVVPGDPAKGFENKAMEAAAKTWTGEW
WKFGGGGTVWHAMAYDAKYDRIYLGTGNGWPWNQKIRSPGGGDNLYLASIVAIDVKTGEY
AWHYQVNPENSHDFNDAMDIELADIEIGGKMRSVLMHAPKNGFFYALDRETGKFISAGEF
AKQNWAKRIDPVTGRPEINPEAQYPNGKPFMMYPFPNGAHGVQAMAFSPKTNLSYIPVME
GGRVFVDPANVKDWTYKPGMMVNTGLGAPPANLVPPAAVSKLVAFDVANNKVAWSVPQPG
VFNGGIMATGGGLLFQGTNDGNFNAYSATDGRKLWSFPAQNGILSAPISYTVGGKQYVSV
ITGFRSSFPNVPNWDYRQQQRRVLTFAIGGTQALPKFEPVDEPIQDDPAFTVDAAKAKVG
GGIYNSSCVICHGAGMMAGGAAPDLRKSAVPLDAETFKSVVHDGALMARGMGAFEQLSDA
DLEGLRHYIRQRARETAPKAN