Protein Info for GFF1392 in Variovorax sp. SCN45

Annotation: Monooxygenase, flavin-binding family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 489 transmembrane" amino acids 236 to 256 (21 residues), see Phobius details PF13450: NAD_binding_8" amino acids 11 to 60 (50 residues), 27.1 bits, see alignment 8.1e-10 PF00743: FMO-like" amino acids 82 to 221 (140 residues), 42.1 bits, see alignment E=8.3e-15

Best Hits

Swiss-Prot: 53% identical to ETHA_MYCS2: FAD-containing monooxygenase EthA (ethA) from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

KEGG orthology group: None (inferred from 72% identity to pla:Plav_0853)

MetaCyc: 59% identical to ethionamide monooxygenase EthA (Mycobacterium tuberculosis H37Rv)
1.14.13.-; 1.14.13.-; 1.14.13.-

Predicted SEED Role

"monooxygenase, flavin-binding family"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (489 amino acids)

>GFF1392 Monooxygenase, flavin-binding family (Variovorax sp. SCN45)
VLQEEHVDVLIVGAGLSGIGAGYHLQASCPGRSYAILEGRERSGGTWDLFRYPGIRSDSD
MYTLGYSFRPWTQAKAIADGPSILKYVRETAQAHGIDKKIRFGHRVVHASWSTPDACWQV
DIEHGPGREPLRMSCNFLFMCSGYYNYAAGYTPEFAGTADYTGRIVHPQHWPDDLDWAGK
RVIVIGSGATAMTLVPALAKTAAHVTLLQRSPTYVVSRPAEDLIANRLRRHLPDRLAYGI
TRWKNVLLSMFFFRLARRKPERVKKMILGGVRQALGPRYDIATHFTPRYNPWDQRICLVP
DGDLFAALNAGRASMVTDRIDRFTPGGIRLESGKELEADIVVTATGLDLQVLGGIALDVD
GVPVDLAAAFNYKGMMYSEVPNLASSFGYTNASWTLKCDLTCEYVCRLLNHMDKHGWRQC
MPRNTDPALAAEPWLDFSSGYVQRSIGKFPKQGSRAPWRLYQNYARDLMSLRFGRVDDGV
MMFEKSRGT