Protein Info for GFF1364 in Variovorax sp. SCN45

Annotation: diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 574 transmembrane" amino acids 31 to 52 (22 residues), see Phobius details amino acids 316 to 335 (20 residues), see Phobius details PF00672: HAMP" amino acids 333 to 384 (52 residues), 38.2 bits, see alignment 1.5e-13 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 409 to 569 (161 residues), 157.4 bits, see alignment E=1.4e-50 PF00990: GGDEF" amino acids 414 to 567 (154 residues), 142.7 bits, see alignment E=9e-46

Best Hits

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (574 amino acids)

>GFF1364 diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s) (Variovorax sp. SCN45)
MSPAHSANDDNHEPPSPARRKGWLRSFRTQVALGFGGLAAVLAVTLSLVLGSMFARKSEA
DESAVLRTIARNASKALADGLTSRTREIELLAASTTLWKDGLGAERVIQTISRTQALTPY
SAWIGVVSPDGVVQASTGKLLLGANVAERPWFQEGLRGTHVGDVHAAKLLAALLPKGSDG
GPQRFVDFAAPIKREGQLIGVLGMHGSWEWTRSVVESLFPDDAVARRLEVFVLDAKDQVI
YASDASFDRSADLGVQPAATGGSGVKFARESTGGEFLMATASVGATDGKIDLGWTVVARE
PADVARAAARDGVKRSLMLGVLGAALAFVLGWLLAQRLTRPLRQIASVARDIGAGRLDTS
IPTTAGSSEVKQLSLALAGMTAKLVSANAELEQRVKERTAALETANAELDRQARFDALTG
LLNRHGMEERLQQALAVSNPDTAPLGIVMIDIDHFKSVNDRFGHATGDLVLKAVAGLMKS
RLRKSDIAARLGGEEFVLMLPGANAAEAMQTATDLVRLVGEAFIDPVGHITISCGVSQML
REDDSVASALRRADKALYAAKSLGRNRAMAPPLS