Protein Info for GFF1354 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 583 PF20423: AceK_regulatory" amino acids 10 to 309 (300 residues), 370.7 bits, see alignment E=5.6e-115 PF06315: AceK_kinase" amino acids 310 to 564 (255 residues), 402.7 bits, see alignment E=7.3e-125

Best Hits

Swiss-Prot: 100% identical to ACEK_SALNS: Isocitrate dehydrogenase kinase/phosphatase (aceK) from Salmonella newport (strain SL254)

KEGG orthology group: K00906, isocitrate dehydrogenase kinase/phosphatase [EC: 2.7.11.5 3.1.3.-] (inferred from 95% identity to cko:CKO_03904)

MetaCyc: 93% identical to isocitrate dehydrogenase kinase/phosphatase (Escherichia coli K-12 substr. MG1655)
3.1.3.-; [Isocitrate dehydrogenase (NADP(+))] kinase. [EC: 2.7.11.5]

Predicted SEED Role

"Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-)" (EC 2.7.11.5, EC 3.1.3.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-

Use Curated BLAST to search for 2.7.11.5 or 3.1.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (583 amino acids)

>GFF1354 Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQQAMKSRIHLYDHHVGL
VVEQLRCITDGKSTDADFLLRVKEHYTRLLPDYPRFEIAESFFNSVYCRLFDHRSLTPER
LFIFSSQPERRFRTIPRPLAKDFFPDHGWEPLLMRILSDLPLRLPWQNKSRDIRYIIAHL
TETLGEDALPRCHVQVANELFYRNKAAWLVGKLTTPDGTLPFLLPIHRTDEGELFVDTCL
TTTAEASIVFGFARSYFMVYAPLPAALVEWLREILPGKTTAELYMAIGCQKHAKTESYRE
YLCYLAESDEKFIEAPGIRGMVMLVFTLPGFDRVFKIIKDKFAPQKEMSAAHVRACYQLV
KEHDRVGRMADTQEFENFVLDKRQIDPALMALLRQEAPEKITDLGEHIVIRHLYIERRMV
PLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEIC
YMTEVNFRDIPPARYPEDELASEPWYSVSPGDVFPEEFRHWLCADPRIGPLFEEMHADLF
RADYWRALQTRIKEGHVEDVYAYRRRQRFSVRYGAISSTANSS