Protein Info for GFF135 in Sphingobium sp. HT1-2

Annotation: Propionyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00289: Biotin_carb_N
  • amino acids 3 to 111 (109 residues), 148.8 bits, E=3e-47
• PF02786: CPSase_L_D2
  • amino acids 116 to 327 (212 residues), 257.9 bits, E=2.7e-80
• PF02222: ATP-grasp
  • amino acids 132 to 281 (150 residues), 28.4 bits, E=4.9e-10
• PF07478: Dala_Dala_lig_C
  • amino acids 136 to 279 (144 residues), 29.9 bits, E=1.6e-10
• PF02785: Biotin_carb_C
  • amino acids 341 to 453 (113 residues), 117 bits, E=1.7e-37
• PF18140: PCC_BT
  • amino acids 473 to 591 (119 residues), 88.9 bits, E=1.3e-28
• PF00364: Biotin_lipoyl
  • amino acids 602 to 665 (64 residues), 59.1 bits, E=1.2e-19
• PF13533: Biotin_lipoyl_2
  • amino acids 603 to 632 (30 residues), 25.1 bits, see alignment (E = 4.9e-09)

Best Hits

KEGG orthology group: K01965, propionyl-CoA carboxylase alpha chain [EC: 6.4.1.3] (inferred from 92% identity to sjp:SJA_C1-17020)

Predicted SEED Role

"Propionyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.3)" in subsystem Propionyl-CoA to Succinyl-CoA Module (EC 6.4.1.3)

MetaCyc Pathways

• propanoyl CoA degradation I (3/3 steps found)
• 3-hydroxypropanoate cycle (10/13 steps found)
• 2-oxobutanoate degradation I (3/4 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (7/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (12/17 steps found)
• methylaspartate cycle (13/19 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (12/18 steps found)
• superpathway of the 3-hydroxypropanoate cycle (12/18 steps found)
• superpathway of L-methionine salvage and degradation (8/16 steps found)
• mycolate biosynthesis (23/205 steps found)
• superpathway of mycolate biosynthesis (24/239 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.3

Use Curated BLAST to search for 6.4.1.3

Protein Sequence (666 amino acids)

>GFF135 Propionyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.3) (Sphingobium sp. HT1-2)
MPITKILIANRGEIACRVMRTAKKMGIKTVAVYSDADARAPHVLMADEAVHIGPPPAAQS
YLLADKIIEACKATGADAVHPGYGFLSERESFRKALDAEGIIFVGPPANAIAAMGDKIES
KKLAMEAGVNVVPGFVGVIDDTEHAVRISNEIGYPVMMKASAGGGGKGMRLAYSEQDVRE
GFEATKREGLNSFGDDRVFIEKFIESPRHIEIQVLGDQHGNIVYLNERECSIQRRHQKVV
EEAPSPFVSAEMRKKMGEQCVALARAVGYFSAGTVELIVSGEDKTGDGFYFLEMNTRLQV
EHPVTEEITGVDLVEQMIRVANGEKLSFTQADVKINGWSIENRVYAEDPYRGFLPSTGRL
IRYNPPETGTDESGALIRVDDGVQEGGEVSIFYDPMIAKLITWAPTRLEAIDKQIEALDK
FEIEGPGHNIDFVSALMQHERFRSGNITTGFIAEEYPEGFTGAPASAELLQRLSAIGAFA
AMAQADRARRIDGQLGKRLAPPTGWQVKIGDAIHDVVIDGDEVTVDGESIDMALEYTPGD
RLIEAEFGEKQLAVRIAPVRSGFVLTAHGASHKLRILPAHAAPYAAHMIEKIPPDLSKYL
ICPMPGLLVALNVKQGDKVEIGQPLAVIEAMKMENILRAGKAGTVKSVSAAQGESLPVDA
VILELE