Protein Info for GFF1342 in Xanthobacter sp. DMC5

Annotation: Pyrophosphatase PpaX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 239 PF00702: Hydrolase" amino acids 16 to 193 (178 residues), 95.9 bits, see alignment E=8.5e-31 PF13419: HAD_2" amino acids 18 to 199 (182 residues), 111.1 bits, see alignment E=1.4e-35 PF12710: HAD" amino acids 18 to 189 (172 residues), 33.8 bits, see alignment E=9.6e-12 TIGR01549: HAD hydrolase, family IA, variant 1" amino acids 79 to 193 (115 residues), 43.3 bits, see alignment E=5.1e-15 PF13242: Hydrolase_like" amino acids 155 to 223 (69 residues), 48.1 bits, see alignment E=1.7e-16

Best Hits

KEGG orthology group: K01091, phosphoglycolate phosphatase [EC: 3.1.3.18] (inferred from 77% identity to xau:Xaut_4762)

Predicted SEED Role

"Similar to phosphoglycolate phosphatase, clustered with ribosomal large subunit pseudouridine synthase C" in subsystem 2-phosphoglycolate salvage

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.18

Use Curated BLAST to search for 3.1.3.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (239 amino acids)

>GFF1342 Pyrophosphatase PpaX (Xanthobacter sp. DMC5)
MSAAADAEPSSSAGLKLVLFDCDGTLVDSQHLIVSAMHDVHAAHGVPVPEREKLLSLVGL
SLPQAFAVLSQGDASYPIEAMVDAYKVAFNRLLNADAPEPMFDGAREVLEGLRQREDVVL
GIVTGKSRRGVNRILSVQGMEGWFSTIQTADDAPSKPHPAMVFQAMGEIGARPEDTVVIG
DTTYDISMALQAGASALGVAWGYHEVPALERAGADRIVQHFREVPEAIAELLARRRVVI