Protein Info for GFF1342 in Variovorax sp. SCN45

Annotation: Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00378: ECH_1
  • amino acids 10 to 194 (185 residues), 115.4 bits, E=7e-37
• PF16113: ECH_2
  • amino acids 13 to 161 (149 residues), 75.9 bits, E=1e-24
• PF02737: 3HCDH_N
  • amino acids 302 to 477 (176 residues), 209.5 bits, E=9.4e-66
• PF00725: 3HCDH
  • amino acids 482 to 574 (93 residues), 83.7 bits, E=2.7e-27
  • amino acids 610 to 698 (89 residues), 29.4 bits, E=2.4e-10

Best Hits

KEGG orthology group: K07516, 3-hydroxyacyl-CoA dehydrogenase [EC: 1.1.1.35] (inferred from 96% identity to vap:Vapar_2925)

Predicted SEED Role

"Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3)" in subsystem Acetyl-CoA fermentation to Butyrate or Isoleucine degradation or Polyhydroxybutyrate metabolism or Valine degradation or n-Phenylalkanoic acid degradation or Butanol Biosynthesis (EC 1.1.1.35, EC 4.2.1.17, EC 5.1.2.3, EC 5.3.3.8)

MetaCyc Pathways

• oleate β-oxidation (29/35 steps found)
• phenylacetate degradation I (aerobic) (8/9 steps found)
• adipate biosynthesis (5/5 steps found)
• adipate degradation (5/5 steps found)
• superpathway of phenylethylamine degradation (9/11 steps found)
• benzoyl-CoA degradation I (aerobic) (6/7 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• benzoyl-CoA biosynthesis (3/3 steps found)
• fatty acid salvage (5/6 steps found)
• 4-hydroxybenzoate biosynthesis III (plants) (4/5 steps found)
• fatty acid β-oxidation III (unsaturated, odd number) (1/1 steps found)
• fatty acid β-oxidation I (generic) (5/7 steps found)
• 2-methyl-branched fatty acid β-oxidation (10/14 steps found)
• L-isoleucine degradation I (4/6 steps found)
• propanoate fermentation to 2-methylbutanoate (4/6 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
• oleate β-oxidation (thioesterase-dependent, yeast) (1/2 steps found)
• valproate β-oxidation (6/9 steps found)
• (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (3/5 steps found)
• fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
• fatty acid β-oxidation IV (unsaturated, even number) (3/5 steps found)
• glutaryl-CoA degradation (3/5 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (18/26 steps found)
• L-valine degradation I (5/8 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)
• oleate β-oxidation (reductase-dependent, yeast) (1/3 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (6/10 steps found)
• methyl ketone biosynthesis (engineered) (3/6 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (5/9 steps found)
• fatty acid β-oxidation V (unsaturated, odd number, di-isomerase-dependent) (2/5 steps found)
• oleate β-oxidation (isomerase-dependent, yeast) (1/4 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
• pyruvate fermentation to butanoate (3/7 steps found)
• 3-phenylpropanoate degradation (5/10 steps found)
• 6-gingerol analog biosynthesis (engineered) (2/6 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (4/9 steps found)
• glycerol degradation to butanol (9/16 steps found)
• benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (1/5 steps found)
• pyruvate fermentation to butanol I (3/8 steps found)
• L-tryptophan degradation III (eukaryotic) (8/15 steps found)
• gallate degradation III (anaerobic) (5/11 steps found)
• 9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) (4/10 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (6/13 steps found)
• 2-methylpropene degradation (2/8 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (8/17 steps found)
• methyl tert-butyl ether degradation (2/10 steps found)
• L-glutamate degradation VII (to butanoate) (3/12 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (6/17 steps found)
• crotonate fermentation (to acetate and cyclohexane carboxylate) (5/16 steps found)
• 10-cis-heptadecenoyl-CoA degradation (yeast) (2/12 steps found)
• 10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) (2/12 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
• toluene degradation VI (anaerobic) (4/18 steps found)
• cholesterol degradation to androstenedione I (cholesterol oxidase) (2/17 steps found)
• androstenedione degradation I (aerobic) (7/25 steps found)
• Spodoptera littoralis pheromone biosynthesis (4/22 steps found)
• platensimycin biosynthesis (6/26 steps found)
• cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (3/22 steps found)
• superpathway of testosterone and androsterone degradation (7/28 steps found)
• androstenedione degradation II (anaerobic) (5/27 steps found)
• superpathway of cholesterol degradation I (cholesterol oxidase) (9/42 steps found)
• superpathway of cholesterol degradation II (cholesterol dehydrogenase) (10/47 steps found)
• superpathway of cholesterol degradation III (oxidase) (6/49 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Bile acid biosynthesis
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Caprolactam degradation
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Limonene and pinene degradation
• Lysine degradation
• Propanoate metabolism
• Tryptophan metabolism
• Valine, leucine and isoleucine degradation
• alpha-Linolenic acid metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.35, 4.2.1.17, 5.1.2.3, 5.3.3.8

Use Curated BLAST to search for 1.1.1.35 or 4.2.1.17 or 5.1.2.3 or 5.3.3.8

Protein Sequence (701 amino acids)

>GFF1342 Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3) (Variovorax sp. SCN45)
MTAEYKVLGDVAVITMTNPPVNGLGFSTRIGITDGLAKANADDAVKAIVITGAGKAFSGG
ADIKEFGTPKALQEPNLLSVILALEASAKPVVAAIHSVCMGGGLELALGCHYRVAAPGTS
VALPEVKLGLIPGAGGTQRLPRVIGVETALNMIVSGEAVKSELLASLPGQKLFDKLAASP
ESVFDEAVAFAKSVAGKTGEALPLVRNLPCKHPQGDAYFQFAKNMVGGMSKNYPAPLQCV
DAVQAATKLKFDAGMAEERRIFTALMFTPESLALRHLFMAERAASKIPDVPEDTPKREIK
AVGVIGAGTMGGGISMNFLNAGIPVKILEMKQEALDRGVATIKKNYEAQVKKGKLKQDKY
EQRMALLSTTLSYDDLKDADLIIEAVFEELGVKEKVFKELDRVAKKGAILASNTSTLDVD
KIAAFTDRPQDVVGMHFFSPANVMKLLEVVRGKATAKDVLATVMAIGKKIKKTAVVSGVC
DGFIGNRMIEQYSRQAGFLLDEGATPQQVDKAIEKFGFAMGPFRMGDLAGNDIGWAIRKR
RAVERADMKYSRTADKLCELGRFGQKTGAGWYDYQAGKRDAIPSDLVNKMIEDHRKELGI
TPRKISDEEIVQRLVYALVNEGAHILEDGIASKSGDIDMVYLTGYGFPIWRGGPMHYASQ
VGLYNVAETMKRFAKNPRDDAEFWQPAPLIQKLVAEGKQFS