Protein Info for GFF1335 in Variovorax sp. SCN45

Annotation: 3-methylmercaptopropionyl-CoA ligase (EC 6.2.1.44) of DmdB2 type

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00501: AMP-binding
  • amino acids 19 to 401 (383 residues), 222.8 bits, E=6.5e-70
• PF13193: AMP-binding_C
  • amino acids 455 to 529 (75 residues), 56.1 bits, E=5.7e-19

Best Hits

KEGG orthology group: K00666, fatty-acyl-CoA synthase [EC: 6.2.1.-] (inferred from 95% identity to vap:Vapar_2932)

Predicted SEED Role

"3-methylmercaptopropionyl-CoA ligase (DmdB)"

KEGG Metabolic Maps

• Alkaloid biosynthesis II
• Benzoate degradation via CoA ligation
• Biosynthesis of plant hormones
• Caprolactam degradation
• Ethylbenzene degradation
• Geraniol degradation
• Limonene and pinene degradation
• Propanoate metabolism
• Tyrosine metabolism
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.-

Use Curated BLAST to search for 6.2.1.-

Protein Sequence (547 amino acids)

>GFF1335 3-methylmercaptopropionyl-CoA ligase (EC 6.2.1.44) of DmdB2 type (Variovorax sp. SCN45)
MLGLMQDQPLLISSLIEFAERHNGDGEIVSRRVEGDIHRTTWGGIASRARQVANALDGEQ
LLFSDRVATLAWNGYRHLELYYGVSGSGRVLHTINPRLHPDQIAWIANHAEDQILCFDLS
FLPLVQAVHARCPMIRKWVALCDADKLPADSGVPNLVSYETWLGQQSTDYDWPTFDENSA
SSMCYTSGTTGNPKAALYSHRSTMLHAYAAALPDVMRISAQDSVLPVVPMFHVNAWGIPY
SAALVGCKLVFPGPSLDGKSVYELIESEGVTFAAGVPTVWQMMLGHMQTNGLKFSKLNRT
VIGGSACPPAMITAFQEKYNVEVLHAWGMTEMSPLGTLCTLKNKHLSLPADAQLQIRMKQ
GRAIFGVDMKIVDGDGNELPWDGKAYGDLLVKGPWVVKEYFKGEGGDPLIADAQGRGWFP
TGDVATIDADGYLQITDRSKDVIKSGGEWISSIDIENIAVAHPAVAMAACIGVFHPKWDE
RPIIAVVKKPGAEVTREELLKFYEGKTAKWQIPDDVVFVEAIPLGATGKILKTRLRELLK
DYKLPTL