Protein Info for GFF1334 in Variovorax sp. SCN45

Annotation: Cocaine esterase (EC 3.1.1.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 628 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details PF02129: Peptidase_S15" amino acids 67 to 316 (250 residues), 114 bits, see alignment E=1e-36 TIGR00976: hydrolase CocE/NonD family protein" amino acids 67 to 559 (493 residues), 159 bits, see alignment E=1.2e-50 PF08530: PepX_C" amino acids 342 to 584 (243 residues), 118.7 bits, see alignment E=3.6e-38

Best Hits

KEGG orthology group: K06978, (no description) (inferred from 77% identity to del:DelCs14_3464)

Predicted SEED Role

"Cocaine esterase (EC 3.1.1.-)" (EC 3.1.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.-

Use Curated BLAST to search for 3.1.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (628 amino acids)

>GFF1334 Cocaine esterase (EC 3.1.1.-) (Variovorax sp. SCN45)
MIALKPSRRWRLAASLLATAAGIAACGGGSSGSGFVFFPQAAETPAPPPSTPDAGPVRKW
GYITLPDGNKMRYSLLLPSATGSFPLLVEYDGYSSGSAPNIGQLWLSEGYAVMGLNVPGT
GCSTGEDQVFDASTGAAGAFAIEWAGKQPWSNGKIGMVGYSYSGYNQLWVAAHRPKGLLA
ITPSKNVGDPYRDVGYPGGIKNIGFPSQWWGGFPTTWKKAADLAAKLDGDTECAKTVADN
IEKIKRPDLDLTKWLENPYADGLYVKRSAALETAKIDIPALGTQSWQDEQIGPRSGYYED
TMAPEKMWLISANGDHHTNVTSDYIDGTLKRFLAHFVKGENNGFEKEPHVRLLQEMQQTD
QKAVNGAAKLKQMAVAEFDRLPVKVKPMRLYLQQGGVLGSAAPSAGSAASSYDYPVKSPV
VNNPAAEGWQAVADTDGQLTFTTAALPEDLSFYGEGSADLFIEATAANGNTDLQVTVSEV
RPDGKEMFVQRGWLRASKRSLDSARSTVLRPFGDFARDTDAPLLAGQPNSVRVEIQKFAH
VFRAGSSIRVTIDTPSQTGFWLFGNDPTPSTNKVWHDATRPSSIVLGYTPYAHAKDLPSC
DRTIRQPCRPAIGAVPAGVGPAAPKDPT