Protein Info for GFF1322 in Variovorax sp. SCN45

Annotation: [Protein-PII] uridylyltransferase (EC 2.7.7.59) / [Protein-PII]-UMP uridylyl-removing enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 868 TIGR01693: protein-P-II uridylyltransferase" amino acids 35 to 866 (832 residues), 766.6 bits, see alignment E=1.9e-234 PF01909: NTP_transf_2" amino acids 65 to 100 (36 residues), 21.3 bits, see alignment (E = 7.2e-08) PF08335: GlnD_UR_UTase" amino acids 171 to 310 (140 residues), 137.1 bits, see alignment E=1.1e-43 PF01966: HD" amino acids 472 to 540 (69 residues), 30 bits, see alignment E=1.4e-10

Best Hits

Swiss-Prot: 59% identical to GLND_BURL3: Bifunctional uridylyltransferase/uridylyl-removing enzyme (glnD) from Burkholderia lata (strain ATCC 17760 / DSM 23089 / LMG 22485 / NCIMB 9086 / R18194 / 383)

KEGG orthology group: K00990, [protein-PII] uridylyltransferase [EC: 2.7.7.59] (inferred from 97% identity to vpe:Varpa_3203)

Predicted SEED Role

"[Protein-PII] uridylyltransferase (EC 2.7.7.59)" in subsystem Ammonia assimilation (EC 2.7.7.59)

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.59

Use Curated BLAST to search for 2.7.7.59

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (868 amino acids)

>GFF1322 [Protein-PII] uridylyltransferase (EC 2.7.7.59) / [Protein-PII]-UMP uridylyl-removing enzyme (Variovorax sp. SCN45)
VIEAAKIDVATLREAYRAKKLALFDTLRFARAPTRSVHTVLRQLAALADETLCTLWREAD
FGDALALAAVGGFGRGELFPYSDVDVLLLLPPEGHENAVDPARIEAFIGHCWDAGLEIGS
SVRTVDECLAEAEKDVTVQTSLLEARLIAGDKKLFTAFRRRFARAIDPQAFFVAKSQEMR
HRHQKFDNTPYALEPNCKESPGGLRDLQTILWMTKAAGYGSRWDDLAKNGLATSFEAQQI
KRNEALLSLIRARLHVIANRREDRLVFDLQTAVAASFGYESESQRKSSEALMRRYYWAAK
AVSQLNQILLLNISERLQPSDERHTPINERFYERAGLIEIASDDLYEREPHAVLETFLLY
QKTIGVKGLSARTLRALYNARHVMDSKFRNDPVNHETFMRILLQPYGITHAFRLMNQTSV
LGRYLRVFRSIVGQMQHDLFHVYTVDQHILMVLRNVRRFFIAEHAHEYPFCSQLAAGWDK
PWILYVAALFHDIAKGRGGDHSTLGARDVQRFCKQHGIAREDAKLIEFLVAEHLVMSQVA
QKQDLSDPEVIGAFAKRVGNERYLTALYLLTIADIRGTSPRVWNAWKGKLLEDLYRYTLR
ALGGRMPDPDAEVEARKREALVQLALHAQRFEAHKALWDTLDVGYFMRHDATEIAWHAKQ
LSRFVPPKGVPIDPKAPPIVRAHLSPVGEGLQVVVYTPDQPDLFARICGYFDQSSFSILD
AKVHTTSNGYALDTFQVVTTFLPDHYRDLISMVESGLGQTLTEAGALPQPSMGRVSRRVR
SFPIKPRISLLPDDKAQRWLLNISASDRAGLLYSVARVLARHHLNLQLAKVTTLGERVED
TFLISGPELQGQKTQLAIETELMEVLAS