Protein Info for GFF1316 in Xanthobacter sp. DMC5

Annotation: Methanol dehydrogenase [cytochrome c] subunit 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details transmembrane" amino acids 490 to 508 (19 residues), see Phobius details amino acids 547 to 565 (19 residues), see Phobius details TIGR03075: PQQ-dependent dehydrogenase, methanol/ethanol family" amino acids 17 to 555 (539 residues), 715.8 bits, see alignment E=1.7e-219 PF13360: PQQ_2" amino acids 61 to 217 (157 residues), 54.8 bits, see alignment E=1.7e-18 PF01011: PQQ" amino acids 82 to 114 (33 residues), 20.6 bits, see alignment (E = 4.3e-08) amino acids 135 to 170 (36 residues), 31.7 bits, see alignment 1.4e-11 amino acids 501 to 534 (34 residues), 21 bits, see alignment (E = 3.2e-08) PF13570: PQQ_3" amino acids 479 to 519 (41 residues), 26.1 bits, see alignment 1.3e-09

Best Hits

Swiss-Prot: 65% identical to XOXF_PARDE: Putative dehydrogenase XoxF (xoxF) from Paracoccus denitrificans

KEGG orthology group: None (inferred from 78% identity to xau:Xaut_4686)

Predicted SEED Role

"Methanol dehydrogenase large subunit protein (EC 1.1.99.8)" in subsystem Respiratory dehydrogenases 1 (EC 1.1.99.8)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.8

Use Curated BLAST to search for 1.1.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (600 amino acids)

>GFF1316 Methanol dehydrogenase [cytochrome c] subunit 1 (Xanthobacter sp. DMC5)
MTRLLPALLLAVLPVAAHANDDVLKRTATGAEQVLQTVDYANTRFSPLRQIDAGNVGRLQ
VAWTFSTGVLRGHEGAPLVVGDVMYVHTPFPNVVYALDLTNEGRILWKYEPRQKKDVAAV
MCCDLVNRGLAYADGLVFLHQADTTIVALDAKTGAKRWSVANGDPALGASNTATVMPVKD
KLIVGISGAEAGVRGHLSAYDLKGGHLVWRAYSTGPDAEMLFDPEKTTELGRPVGRDSSL
KSWSGDQWKIGGGATWGWFAYDPKLNLVYYGTANPATWNPTQRPGDNRWSDTIIARDADT
GVARWVYQMTPHDEWDYDGVNEMILTEQEVDGKPTPLLTHFDRNGFAYTLNRETGALLRA
GKYETTANWASGIDLDPQSPSYGRPKRDPAFSTEREGEDVTYTGICPSSLGAKNQQPAAF
SPQTRLFYVPVTRMCMDYEPFHVDYVPGQPYVGATLSMHPAPGKEGSTGGFLAWDALKGE
AVWSKPELFSVWSGALATAGGIVFYGTLEGWLKAVDARTGQELYRFKTPSGIVGNVTTFM
QGGRQYVAVLSGVGGWAGIGLAAGLSDPGDGSGANEAYAALNQYTALGGQLTVFALPKGE