Protein Info for GFF1311 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01855: imidazole glycerol phosphate synthase, glutamine amidotransferase subunit
  • amino acids 6 to 217 (212 residues), 190.5 bits, E=1.4e-60
• PF00117: GATase
  • amino acids 8 to 215 (208 residues), 60.8 bits, E=1.4e-20

Best Hits

Swiss-Prot: 62% identical to HIS5_BURM1: Imidazole glycerol phosphate synthase subunit HisH (hisH) from Burkholderia multivorans (strain ATCC 17616 / 249)

KEGG orthology group: K02501, glutamine amidotransferase [EC: 2.4.2.-] (inferred from 75% identity to pol:Bpro_0807)

Predicted SEED Role

"Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-)" in subsystem Histidine Biosynthesis (EC 2.4.2.-)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• L-histidine biosynthesis (10/10 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.-

Use Curated BLAST to search for 2.4.2.-

Protein Sequence (220 amino acids)

>GFF1311 Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MKRKTVAVVDYGSGNLRSVSQAVQHVARAQDVDVLVTARAQDVLDADRVVLPGQGHMGDC
MRELAASGLLDAVLHAAANKPLFGVCVGMQMLLDRSEEGPTDGLGLIPGEVVRFQLEGRL
QADGSRFKVPQMGWNPVFHELGRGPAHALWSGIADGAYFYFVHSFFARPAQAVHSVGEAD
YGGRFAAAIARDNIFATQFHPEKSADQGLALYRNFLSWNP