Protein Info for GFF1300 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Outer membrane stress sensor protease DegS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 382 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details PF00089: Trypsin" amino acids 102 to 264 (163 residues), 71.5 bits, see alignment E=2.4e-23 PF13365: Trypsin_2" amino acids 105 to 239 (135 residues), 122.8 bits, see alignment E=5.1e-39 PF00595: PDZ" amino acids 274 to 347 (74 residues), 39.6 bits, see alignment E=1.4e-13 PF13180: PDZ_2" amino acids 279 to 367 (89 residues), 52.6 bits, see alignment E=1.1e-17 PF17820: PDZ_6" amino acids 304 to 358 (55 residues), 41.5 bits, see alignment 2.2e-14

Best Hits

KEGG orthology group: K01362, [EC: 3.4.21.-] (inferred from 75% identity to pol:Bpro_0817)

Predicted SEED Role

"Outer membrane stress sensor protease DegS" in subsystem Proteolysis in bacteria, ATP-dependent

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (382 amino acids)

>GFF1300 Outer membrane stress sensor protease DegS (Hydrogenophaga sp. GW460-11-11-14-LB1)
MKRLWLLFAQTVTVLVAALFVVNTLQPQWLGRQASIVPVFEAPSGAPVATVEGTGSFRAA
ARTASAAVVSINTSKAPVNNPQSSDPWFRFFFGDQAPSQQQSGLGSGVIVSPAGYVLTNN
HVIEEADEIEVILNDGRKSVAQVIGTDPETDLAILKINLTDLPVITLGNSDALEIGDQVL
AIGNPFGVGQTVTSGIVSALGRTQLGINTFENFIQTDAAINPGNSGGALVDVSGQLMGIN
TAIYSRSGGSMGIGFAIPTSTARSVMEAIVKDGQVTRGWIGVEPQDLSPELAENFGMQPG
TGVIISGVLQNGPAAQAGIRPGDVITAVAGRPVNNVAQLLSAVANLTPGTPASLDVVRRD
GKTKVEVTPGKRNLPRQQATPR