Protein Info for GFF1288 in Pseudomonas sp. DMC3

Annotation: FAD:protein FMN transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 328 PF02424: ApbE" amino acids 24 to 304 (281 residues), 267.3 bits, see alignment E=8.2e-84

Best Hits

KEGG orthology group: K03734, thiamine biosynthesis lipoprotein (inferred from 77% identity to pfo:Pfl01_3863)

Predicted SEED Role

"Thiamin biosynthesis lipoprotein ApbE"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (328 amino acids)

>GFF1288 FAD:protein FMN transferase (Pseudomonas sp. DMC3)
VVLAVAIGGCGTGDSLEQVGGSTMGSTWSVKYVRHAGGADPARVRGEVQAILGEVDQQLS
TYRSDSAIERFNALPADSCQAMPAAVLELVRIGEQLSTQSEGSYDLTVEPLLDLWGFGPQ
GREEKIPDAAAVAEVMQRVGHQHLRIDGEQLCKDAAVEVDFNSIAAGYAVDAIAARLQGM
GIDNYLVEATGELKARGKKPDGSAWRIALEEPRDDQQVAERIIDVDGYGVSTSGDYRHYF
LQDGRRYSHTFDARSGRPVLHDLASVTVIHPSALMADGLSTLLLILGPERARDYAQEHDI
AAFFVLRADTGFVTRSSQAFTQLVGEKN