Protein Info for GFF1287 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Glutamate N-acetyltransferase (EC 2.3.1.35) / N-acetylglutamate synthase (EC 2.3.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00120: glutamate N-acetyltransferase/amino-acid acetyltransferase
  • amino acids 16 to 415 (400 residues), 397.6 bits, E=2.9e-123
• PF01960: ArgJ
  • amino acids 22 to 415 (394 residues), 468.9 bits, E=5.1e-145

Best Hits

Swiss-Prot: 67% identical to ARGJ_RALSO: Arginine biosynthesis bifunctional protein ArgJ (argJ) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K00620, glutamate N-acetyltransferase / amino-acid N-acetyltransferase [EC: 2.3.1.1 2.3.1.35] (inferred from 76% identity to vap:Vapar_4231)

Predicted SEED Role

"Glutamate N-acetyltransferase (EC 2.3.1.35) / N-acetylglutamate synthase (EC 2.3.1.1)" in subsystem Arginine Biosynthesis extended (EC 2.3.1.1, EC 2.3.1.35)

MetaCyc Pathways

• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• superpathway of arginine and polyamine biosynthesis (15/17 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• L-ornithine biosynthesis I (5/5 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.1

Use Curated BLAST to search for 2.3.1.1 or 2.3.1.35

Protein Sequence (415 amino acids)

>GFF1287 Glutamate N-acetyltransferase (EC 2.3.1.35) / N-acetylglutamate synthase (EC 2.3.1.1) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MPVLLNAPLVTDLLPVAGVRIGVAKAGIRKANRKDLTVFLLDEGCAVGAVFTQNRYAAAP
VQVCRDHLASGQSIRAMVINTGNANAGTGEPGLANARQTCNALARSLGLHHEQVLPFSTG
VIMEPLPLDRLLAGLPAALADAAQPQTEGGAQWLSAAEGIMTTDTLPKAFSQRFELDGRT
VTATGIAKGAGMIRPNMATMLGFLATDASVAPALMNALARRLADASFNRVTVDGDTSTND
SFVVVATGKAGNAPITDLDSPAGRALLAALVPVARHLAHAIVRDGEGATKFITVQVEGGR
DASECLLAAYAVAHSPLVKTAFFASDPNLGRILAAVGYAGIHDLDVSGIDLHLGDVQVVN
AGGRHPAYKEEDGQRVMKQAEILVRIGLGRGTASETVWTCDFSHEYVTINADYRS