Protein Info for GFF1277 in Sphingobium sp. HT1-2

Annotation: Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 691 transmembrane" amino acids 47 to 68 (22 residues), see Phobius details PF00912: Transgly" amino acids 102 to 265 (164 residues), 205.2 bits, see alignment E=5.5e-65 TIGR02074: penicillin-binding protein, 1A family" amino acids 104 to 608 (505 residues), 512.8 bits, see alignment E=6.2e-158 PF00905: Transpeptidase" amino acids 347 to 604 (258 residues), 96.8 bits, see alignment E=1.5e-31

Best Hits

KEGG orthology group: None (inferred from 85% identity to sjp:SJA_C1-22810)

Predicted SEED Role

"Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-)" in subsystem Peptidoglycan Biosynthesis or Type IV pilus (EC 2.4.1.129, EC 3.4.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.129, 3.4.-.-

Use Curated BLAST to search for 2.4.1.129 or 3.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (691 amino acids)

>GFF1277 Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-) (Sphingobium sp. HT1-2)
MAVPMTELPNPAPTGTPAPAADEARPFGAGPPSAFAPPTPWKRRLQIFGWIVAAGLALLM
LIIAWLAVTAPMSRSLQPIAPPSISLMSADGHLVARRGAIIDKPVEVAKLPAHVPQAFMA
IEDRRFYDHWGVDPRGIARAMWHNIWSDGSSQGGSTITQQLAKGVFLSSDRTMGRKAREA
LIAFWLEAWLTKDQILERYLSNVYFGDNVYGLRAASLHYFDRQPERLTISQAAMLAGLLK
APSRLAPTSNLKGARARAALVTQAMVEAGYISKAQRDQLAPARLDVRESPDATSGTYFAD
WVLPEARDRAGAVYGAQEVKTTLDWRIQRLAEAAIRRAPLGGAQVALVAMKPDGSVVAMI
GGKNYEKSSFNRAVQAKRQPGSTFKLFVYLAAFRAGMTPDDMIEDTPITTGTYRPANHGG
KYRGRITLRQAFAASSNVAAVRLTQKVGVDNVIKVARDLGVTAPLTEDLSLALGTSEIPL
VELAEAYASVAAGAYPVLAHGLPPEEQGWFDRLMKRQRHFSDDQLEMIRDLLSSAANRGT
GSAAALRTSTFGKTGTTQDSRDAIFVGYAGGLVTAVWIGNDDNAPLPGGAAGGGVPARIW
RNFMAGAINEPVEKVPEEEQDPDLVNAIANVSVETGIGNVGVGMDQGGMTVTIDGNQLRI
PSDQPLPLPPQGVPPPRIAPTQPQENEPPQP