Protein Info for GFF1264 in Methylophilus sp. DMC18

Annotation: Phosphate acyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02504: FA_synthesis
  • amino acids 12 to 334 (323 residues), 368 bits, E=4e-114
• TIGR00182: fatty acid/phospholipid synthesis protein PlsX
  • amino acids 14 to 342 (329 residues), 380.4 bits, E=4e-118
• PF01515: PTA_PTB
  • amino acids 84 to 223 (140 residues), 25.9 bits, E=6.4e-10

Best Hits

Swiss-Prot: 75% identical to PLSX_METFK: Phosphate acyltransferase (plsX) from Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)

KEGG orthology group: K03621, glycerol-3-phosphate acyltransferase PlsX [EC: 2.3.1.15] (inferred from 82% identity to mmb:Mmol_1146)

Predicted SEED Role

"Phosphate:acyl-ACP acyltransferase PlsX" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria

MetaCyc Pathways

• phosphatidylglycerol biosynthesis I (6/6 steps found)
• phosphatidylglycerol biosynthesis II (6/6 steps found)
• superpathway of phospholipid biosynthesis III (E. coli) (10/12 steps found)
• CDP-diacylglycerol biosynthesis I (4/4 steps found)
• CDP-diacylglycerol biosynthesis II (4/4 steps found)
• oleate biosynthesis III (cyanobacteria) (2/3 steps found)
• phosphatidate biosynthesis (yeast) (3/5 steps found)
• superpathway of cardiolipin biosynthesis (bacteria) (8/13 steps found)
• diacylglycerol and triacylglycerol biosynthesis (3/7 steps found)
• palmitoyl ethanolamide biosynthesis (2/6 steps found)
• superpathway of stearidonate biosynthesis (cyanobacteria) (2/6 steps found)
• stigma estolide biosynthesis (2/7 steps found)
• anandamide biosynthesis II (2/8 steps found)
• anandamide biosynthesis I (2/12 steps found)
• superpathway of phospholipid biosynthesis II (plants) (10/28 steps found)

KEGG Metabolic Maps

• Glycerolipid metabolism
• Glycerophospholipid metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.15

Use Curated BLAST to search for 2.3.1.15

Protein Sequence (350 amino acids)

>GFF1264 Phosphate acyltransferase (Methylophilus sp. DMC18)
MTTGQHVHSHEVVIAIDAMGGDHGPHVTVPAAVRILNENPELNIILVGLQEAIESELKVL
NVQPGPRLRIRHASEVVLMDESPQSAMKNKKDSSMRVAINMVKTGEAQACVSAGNTGALM
ATARFVLKTLPGIDRPAIASTLPSEKGSTFMLDLGANADCTPEHLYQFAIMGAMVVSCLT
NKPNPTVGLLNIGSEDIKGNEVVKQAAELLRGSHLNFYGNVEGDDIFKGTTDVVVCDGFV
GNVSLKTTEGLAHMMGKFLTQEFKRNWLTKLMALGAFPVLKAFKKRVDPRLYNGASFLGL
RGIVVKSHGGADSVGFYHAIHVAMEEAQSGVLKRIQSQLEIEHLTTSEQP