Protein Info for GFF1256 in Variovorax sp. SCN45

Annotation: Probable NADH dehydrogenase/NAD(P)H nitroreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00881: Nitroreductase
  • amino acids 18 to 173 (156 residues), 63.4 bits, E=1.5e-21

Best Hits

Swiss-Prot: 62% identical to Y554_XANAC: Putative NADH dehydrogenase/NAD(P)H nitroreductase XAC0554 (XAC0554) from Xanthomonas axonopodis pv. citri (strain 306)

KEGG orthology group: K09019, putative NADH dehydrogenase/NAD(P)H nitroreductase RutE [EC: 1.-.-.-] (inferred from 93% identity to vpe:Varpa_2676)

MetaCyc: 50% identical to FAD reductase (NADH) (Cupriavidus nantongensis)
RXN-8506 [EC: 1.5.1.37]

Predicted SEED Role

"Oxygen-insensitive NADPH nitroreductase (EC 1.-.-.-)" (EC 1.-.-.-)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Carotenoid biosynthesis - General
• Insect hormone biosynthesis
• Nucleotide sugars metabolism
• Porphyrin and chlorophyll metabolism
• Puromycin biosynthesis
• Trinitrotoluene degradation
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.- or 1.5.1.37

Protein Sequence (198 amino acids)

>GFF1256 Probable NADH dehydrogenase/NAD(P)H nitroreductase (Variovorax sp. SCN45)
MTTLNTIPEAALDQLFRKARTVHAFKPVPVTDELIHRLYDLVKWGPTAFNAQPARYVFVR
SEEAKAKLKPAVSAGNTAQTLAAGVTVIVAHDTRFYEHLPTQFPAYNAKPLFEKSAEAAQ
ATAFRNSSLQGAYLILAARSLGLDAGPQSGFNAELVDKAFFPDGRYKANFLVNLGVADHA
GTFERGPRLAFDEVAEIV