Protein Info for GFF1254 in Pseudomonas sp. DMC3

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 869 transmembrane" amino acids 21 to 44 (24 residues), see Phobius details amino acids 255 to 278 (24 residues), see Phobius details PF05228: CHASE4" amino acids 63 to 222 (160 residues), 50.8 bits, see alignment E=7.2e-17 TIGR00229: PAS domain S-box protein" amino acids 310 to 430 (121 residues), 45 bits, see alignment E=1.1e-15 PF13188: PAS_8" amino acids 314 to 375 (62 residues), 27.2 bits, see alignment 9.6e-10 PF00989: PAS" amino acids 314 to 423 (110 residues), 36.9 bits, see alignment E=1.1e-12 PF08448: PAS_4" amino acids 321 to 427 (107 residues), 28.7 bits, see alignment E=4.5e-10 PF13426: PAS_9" amino acids 325 to 425 (101 residues), 32.1 bits, see alignment E=4.1e-11 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 433 to 595 (163 residues), 145 bits, see alignment E=1.7e-46 PF00990: GGDEF" amino acids 438 to 592 (155 residues), 160.2 bits, see alignment E=1.3e-50 PF00563: EAL" amino acids 613 to 844 (232 residues), 243.3 bits, see alignment E=8e-76

Best Hits

KEGG orthology group: None (inferred from 81% identity to pfo:Pfl01_2709)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (869 amino acids)

>GFF1254 hypothetical protein (Pseudomonas sp. DMC3)
MAVTLPAQPIPTPQRAVTRRLAIAVGALFIAGVIAAISTLLGIANTLDAKDLEQNQFYSV
RAVENRITASKNYITSYAYWTRAYEALSGPIDVNWAYTEQNMGKTLFTSDGYDGVFVLDR
NRTKYAVIRGQMVETDVSSVLQAPASALVEQVLSQPDLTKPISRYSLFEGWPALITAAAI
IPNDERPLDELRNTSVLVFVDKLTPTKLRKIGSGYGLTNLTLVPDDTIEKGHPSVPLDST
GYSLVADLQRPGQQLLWTLVPPLGATFLVLMLLTAYFFRHALRSSQYVDNSIHAMQASNQ
ALEAANHALEASEERFRAVAEAASDWIWEVDDQLALTYLSARFSEVTGYPQALWLGQDIG
ELLTCDTTPLELWLRKLSEENSAGDLRCTYRDHSGQPRHCRLSARPIFDKDSVVGYRGTA
SDITDEVAAHAQIQHLSMHDALTGLPNRNKLARYLDEVLILKEHSPPLSLLMIDLDNFKP
INDSLGHPAGDAVLQEVATRLRECTREHDLVARLGGDEFVVVLNGMDSHNEIDKFCTRLI
GSLHQPILYEQHPLHIGASVGIALSRRHGFVASDLIRYADIALYQAKSDGKNTWCYFEAH
MSDQIQTRRQMEDDLRHALKHNEFVLHYQPRYKVDGKHIVSVEALVRWQHPTQGLLGPDV
FIPLAEQTDLIVPLGRWVLREACETALEWPHDILLSVNLSPAQFALSDVVEDVREVLVDT
RFPASRLELEITENVMLNDTDGALTTMNQLKELGVRLNMDDFGTGYSSLGYLRAYPFDGI
KIDKRFIASISSGTNDRAVVQAIIGLGKAMGLTVTAEGVETEEQLAILGKDQCNEVQGYF
MSRPIDKAAFADLLRASRLSLVSKKGRVT