Protein Info for GFF1253 in Pseudomonas sp. DMC3

Annotation: Cytochrome bd-I ubiquinol oxidase subunit 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 333 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details transmembrane" amino acids 71 to 97 (27 residues), see Phobius details amino acids 118 to 141 (24 residues), see Phobius details amino acids 160 to 183 (24 residues), see Phobius details amino acids 198 to 217 (20 residues), see Phobius details amino acids 229 to 247 (19 residues), see Phobius details amino acids 258 to 280 (23 residues), see Phobius details amino acids 298 to 318 (21 residues), see Phobius details PF02322: Cyt_bd_oxida_II" amino acids 7 to 323 (317 residues), 270.8 bits, see alignment E=8.3e-85 TIGR00203: cytochrome d ubiquinol oxidase, subunit II" amino acids 16 to 225 (210 residues), 128.9 bits, see alignment E=1.4e-41

Best Hits

KEGG orthology group: K00426, cytochrome bd-I oxidase subunit II [EC: 1.10.3.-] (inferred from 42% identity to bpa:BPP1025)

Predicted SEED Role

"putative Cytochrome bd2, subunit II" in subsystem Terminal cytochrome d ubiquinol oxidases or Terminal cytochrome oxidases

Isozymes

Compare fitness of predicted isozymes for: 1.10.3.-

Use Curated BLAST to search for 1.10.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (333 amino acids)

>GFF1253 Cytochrome bd-I ubiquinol oxidase subunit 2 (Pseudomonas sp. DMC3)
MDTDWLTLLCAAALAFSVMNYVVLDGTDLGVGMLMGLTPCSKQRRAMAVSILPVWDANET
WLVLGGGGLLALFPLAYAILLPALYVPFIVMFLALIVRAMALEFRDYAPNERRKRIVDGV
LALASLLTGFIQGMVLGTLVQGIAHQGGQYIGDGRDWLSPFPVFCGVVLVLGYVWLGTCW
LYWRTLDELQQRSARQARVLSLVTVALLLVLIAWTATLQPQYARRLADIRLWLPAGLIVS
ALGWVFSKGFHCRFDFIPLFAALGIFVLAFAVMLVALYPFIIPPDLTLSEAASSPTSQIF
MLVGFAVLIPITLAYNTFGFRVFSGKVRVANDG