Protein Info for GFF1252 in Pseudomonas sp. DMC3

Annotation: Cytochrome bd ubiquinol oxidase subunit 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 457 transmembrane" amino acids 15 to 39 (25 residues), see Phobius details amino acids 57 to 78 (22 residues), see Phobius details amino acids 98 to 121 (24 residues), see Phobius details amino acids 128 to 148 (21 residues), see Phobius details amino acids 187 to 208 (22 residues), see Phobius details amino acids 220 to 239 (20 residues), see Phobius details amino acids 326 to 349 (24 residues), see Phobius details amino acids 361 to 382 (22 residues), see Phobius details amino acids 413 to 433 (21 residues), see Phobius details PF01654: Cyt_bd_oxida_I" amino acids 11 to 438 (428 residues), 503.2 bits, see alignment E=2.4e-155

Best Hits

KEGG orthology group: K00425, cytochrome bd-I oxidase subunit I [EC: 1.10.3.-] (inferred from 62% identity to xcc:XCC2662)

MetaCyc: 52% identical to cyanide insensitive ubiquinol oxidase subunit I (Pseudomonas putida KT2440)
RXN-6883 [EC: 1.10.3.11]

Predicted SEED Role

"putative Cytochrome bd2, subunit I" in subsystem Terminal cytochrome d ubiquinol oxidases or Terminal cytochrome oxidases

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.10.3.-

Use Curated BLAST to search for 1.10.3.- or 1.10.3.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (457 amino acids)

>GFF1252 Cytochrome bd ubiquinol oxidase subunit 1 (Pseudomonas sp. DMC3)
MEPVDSALMAARAQFAITISFHIVLAAFSIGLANFLMVLEALWLRTGRQVYLDVYRYWLK
VFALNVAVGTASGLILEYQFGLNWSRLSVQAGDVLGPLMFYEVMAAFFIEAGFLGIMLFG
LKKVGPRLHFFATCAVAVGSLISAFWILSANSWMQTPAGYSIGADGRFLAEDWWAIIFNP
SFPYRLAHMLLAVFIGSATLIAGISAWQLRKARENPRARLQFSMALWVIALLMPVQIVVG
DLHGRHSLHAQPQKVAAIEGSWTRPAQGAGEPLRLFAWPDMQERRNHWEVAIPRIGSMYL
QHDLTSTIAALDEFPAEDIPPVAPVFFAFRLMVGLGLLMLGQGVVSLILRRRNRLYTARR
WLGACVLLAPAGFLAMLSGWVVTEVGRQPFTVYGLMRTADSLSSVSRAQVVGSTWLILLF
YLLIFGIGLWVLLRLLREPPQEDQPGPQPTLADETGH