Protein Info for GFF125 in Variovorax sp. SCN45

Annotation: Dihydroorotase (EC 3.5.2.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 63% identical to PYRC_NOVAD: Dihydroorotase (pyrC) from Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CIP 105152 / NBRC 16084 / F199)

KEGG orthology group: K01465, dihydroorotase [EC: 3.5.2.3] (inferred from 68% identity to bgf:BC1003_5005)

MetaCyc: 61% identical to dihydroorotase (Escherichia coli K-12 substr. MG1655)
Dihydroorotase. [EC: 3.5.2.3]

Predicted SEED Role

"Dihydroorotase (EC 3.5.2.3)" in subsystem De Novo Pyrimidine Synthesis (EC 3.5.2.3)

MetaCyc Pathways

superpathway of histidine, purine, and pyrimidine biosynthesis (43/46 steps found)
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
UMP biosynthesis II (6/6 steps found)
UMP biosynthesis III (6/6 steps found)
superpathway of pyrimidine ribonucleotides de novo biosynthesis (8/9 steps found)
UMP biosynthesis I (5/6 steps found)

KEGG Metabolic Maps

Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.2.3

Use Curated BLAST to search for 3.5.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (350 amino acids)

>GFF125 Dihydroorotase (EC 3.5.2.3) (Variovorax sp. SCN45)
MNQMTSASSITIRRPDDWHLHLRDGQMLRTVLGHTTRQFARAIVMPNLKTPVTTVAQARA
YRERIMAALPPGAHFTPLMTAYLCDGTPPDELRRGFDEGVFTAVKLYPAGATTHSEFGVT
STARVAKALETMQAIGMPLLVHGESTDPDVDIFDREAAFIDRTLRPLLADFPALKVVMEH
ITTEEAADFVSSDASGRLGATLTPQHLMFNRNAIFTGGIRPHLYCLPVLKRERHRLALRR
VSTSGDARFFLGTDSAPHLRMLKEAGCGCAGIFNAPVALQSYLTVFEEEKALERFEAFAS
VNGARFYGLPLNEGTVTLHRTPMDVPENVELLDGAAVHPFLGGSTLPWSL