Protein Info for GFF1245 in Variovorax sp. SCN45

Annotation: tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (EC 2.1.1.61) / FAD-dependent cmnm(5)s(2)U34 oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 613 PF05430: Methyltransf_30" amino acids 107 to 227 (121 residues), 111.3 bits, see alignment E=8.8e-36 TIGR03197: tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain" amino acids 249 to 612 (364 residues), 236.4 bits, see alignment E=3.1e-74 PF01266: DAO" amino acids 250 to 591 (342 residues), 166.2 bits, see alignment E=4.7e-52 PF13450: NAD_binding_8" amino acids 252 to 280 (29 residues), 25.3 bits, see alignment (E = 4.5e-09)

Best Hits

Swiss-Prot: 52% identical to MNMC_DELAS: tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC) from Delftia acidovorans (strain DSM 14801 / SPH-1)

KEGG orthology group: None (inferred from 80% identity to vpe:Varpa_2665)

Predicted SEED Role

"tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (EC 2.1.1.61) / FAD-dependent cmnm(5)s(2)U34 oxidoreductase" (EC 2.1.1.61)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.61

Use Curated BLAST to search for 2.1.1.61

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (613 amino acids)

>GFF1245 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (EC 2.1.1.61) / FAD-dependent cmnm(5)s(2)U34 oxidoreductase (Variovorax sp. SCN45)
LAAEPVTWRADGVPRSERFDDIYHTETGALAQSRHVFLGGCGLPEAWAGQPQWLILETGF
GLGLNFLTTWQAWRADPERPRMLHFVSVEAHPVAPGDLLRGAGAYPELAALAGELAAQWH
GLLPGFHRLSFDDGRVLLTLCIGDVQPMLRAQRFEADSIFLDGFSPQQNPDMWSADTLKA
VSRFARQGTRIATWTIARAIRDALQQYGFAVEKYKGLPPKRDCLRGVFAPAWTVRRREPP
PERIDAPGHCAVIGAGLAGAAVAASLARRGWRVTVLDAADWPASGASGLPVGMLAPHVSP
DDALLSRLTRAGIRVTWTELERLLEDGRDWRASGVLERRPDGDVRVPAGWGASGPNESWP
ASAGQLASTGLPGDTPALWHSRAAWVRPHRLIAAWLRQPGVEFRGGCRVARVELEPGGWQ
LLDPAGQSLAEADRVVICAGHESGRFAPALPLQPVRGQVAWGAMPGSTALPPTPINGDGH
LIAHVPDGPGTQIWLAGATFDRDSTDLAPTATDADANRERLARLHPASAAALAPSFSSGD
VESWVGIRCASGDRRPLVGPLEGSATGLWACTALGSRGLSFAALCAELLAAQWHAEPLPL
PATLAKALGTQRL