Protein Info for GFF1243 in Pseudomonas sp. DMC3

Annotation: Purine ribonucleoside efflux pump NepI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 408 transmembrane" amino acids 16 to 35 (20 residues), see Phobius details amino acids 55 to 75 (21 residues), see Phobius details amino acids 86 to 106 (21 residues), see Phobius details amino acids 112 to 134 (23 residues), see Phobius details amino acids 145 to 165 (21 residues), see Phobius details amino acids 175 to 196 (22 residues), see Phobius details amino acids 216 to 239 (24 residues), see Phobius details amino acids 254 to 276 (23 residues), see Phobius details amino acids 283 to 303 (21 residues), see Phobius details amino acids 309 to 330 (22 residues), see Phobius details amino acids 342 to 363 (22 residues), see Phobius details amino acids 370 to 389 (20 residues), see Phobius details PF07690: MFS_1" amino acids 25 to 356 (332 residues), 121.3 bits, see alignment E=4.4e-39 amino acids 223 to 400 (178 residues), 51.1 bits, see alignment E=1e-17 PF00083: Sugar_tr" amino acids 53 to 193 (141 residues), 29.3 bits, see alignment E=4.3e-11

Best Hits

Swiss-Prot: 45% identical to NEPI_SALG2: Purine ribonucleoside efflux pump NepI (nepI) from Salmonella gallinarum (strain 287/91 / NCTC 13346)

KEGG orthology group: None (inferred from 86% identity to pfs:PFLU2381)

Predicted SEED Role

"major facilitator superfamily MFS_1"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (408 amino acids)

>GFF1243 Purine ribonucleoside efflux pump NepI (Pseudomonas sp. DMC3)
MNERVGDGRTDGEPLVPAWMAVFSLAMGVFGLLTAEYLPASLLTPMALDLGVSEALAGQA
VTVTAVVALFAGLLVPGLTRSLDRRLVLLGFSALMIASNLLVALSSSLAVLLIMRILLGI
ALGGFWSMAAAVAMRLVPAALLPRALSIIFSGIAVGTVVAVPLGSYLGGQFGWRSAFFAA
AAVGVVTLVFQMFTLPSLAPHRPSRLRTVLEVLLRPGIAVGMFGCVLVHTGHFALFTYIR
PFLESTTGVGPQTLALMLLGFGVANFAGTLFAGWLLQRNPRATLVLMPVLVGVAALALVG
LPASVTGQALLLALWGMAFGGVPVAWSNWVARSIPDQAESAGGMVVASVQSAIAAGAAAG
GSVFSFSGINGVFVAAGILMLLAALLIALKVNVDVPEPGSAPVAPVHL