Protein Info for GFF1239 in Xanthobacter sp. DMC5

Annotation: NADH-quinone oxidoreductase subunit L

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 641 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details transmembrane" amino acids 30 to 53 (24 residues), see Phobius details amino acids 74 to 78 (5 residues), see Phobius details amino acids 80 to 101 (22 residues), see Phobius details amino acids 113 to 130 (18 residues), see Phobius details amino acids 136 to 155 (20 residues), see Phobius details amino acids 176 to 199 (24 residues), see Phobius details amino acids 220 to 243 (24 residues), see Phobius details amino acids 253 to 272 (20 residues), see Phobius details amino acids 283 to 304 (22 residues), see Phobius details amino acids 311 to 333 (23 residues), see Phobius details amino acids 339 to 358 (20 residues), see Phobius details amino acids 378 to 400 (23 residues), see Phobius details amino acids 420 to 441 (22 residues), see Phobius details amino acids 461 to 485 (25 residues), see Phobius details amino acids 516 to 538 (23 residues), see Phobius details amino acids 618 to 638 (21 residues), see Phobius details TIGR01974: proton-translocating NADH-quinone oxidoreductase, chain L" amino acids 2 to 637 (636 residues), 882 bits, see alignment E=1.7e-269 PF00662: Proton_antipo_N" amino acids 64 to 114 (51 residues), 81.8 bits, see alignment 3.9e-27 PF00361: Proton_antipo_M" amino acids 130 to 419 (290 residues), 304.9 bits, see alignment E=8.7e-95

Best Hits

KEGG orthology group: K00341, NADH dehydrogenase I subunit L [EC: 1.6.5.3] (inferred from 88% identity to xau:Xaut_4622)

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (641 amino acids)

>GFF1239 NADH-quinone oxidoreductase subunit L (Xanthobacter sp. DMC5)
MYQAIVFLPLLGFLIAGLFGKKIGDRASEVVTTSFLFVACLLSWIAFFSVGFGDHDTRVQ
VMKWIYVGDLKVDWAFRIDTMTVIMLIVVTTVSSLVHLYSIGYMHEDPSRPRFFAYLSLF
TFAMLMLVTADNLLQLFFGWEGVGLASYLLIGFWYEKPSANAAAMKAFVVNRVGDFGFML
GIFAIFVMTGSIAFEEVFAAAPGLAGKTITFLGHHWDAPTVIALLLFVGACGKSAQFLLH
TWLPDAMEGPTPVSALIHAATMVTAGVFMVARMSPIFELSQTALNVVMLVGATTAFFAAT
VGLVQNDIKKVIAYSTCSQLGYMFVAMGAGAYSIGMFHLLTHAFFKALLFLGAGSVIHAM
HHEQDMRHMGGLRKKIPFTYWTMVIGTLAITGFPFLAGYYSKDAIIEAAYASHNYFHVYG
YWMTVIAAALTAFYSWRLIFLTFWGHPHDHHHYEAAHESPLVMTIPLAVLSLGAIFAGWA
FHGIFVGHGVEHFFRHSIFMGAENHILHAMHEIPGWAAYMPTVMMVIGSLFAIWFYLVNP
KVPVQLAKSQDALYQFLLNKWYFDEIYNFLFVRPALWLGRTLWKRGDGTIIDGFGPDGVS
ARVLDITRGVVRLQTGYLYHYAFVMLIGVAALITWFMFAGV