Protein Info for GFF123 in Variovorax sp. SCN45

Annotation: Dihydroorotate dehydrogenase (NAD(+)), catalytic subunit (EC 1.3.1.14)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 316 TIGR01037: dihydroorotate dehydrogenase family protein" amino acids 4 to 297 (294 residues), 334.1 bits, see alignment E=3.4e-104 PF01180: DHO_dh" amino acids 5 to 281 (277 residues), 254.3 bits, see alignment E=2.1e-79 PF01207: Dus" amino acids 92 to 274 (183 residues), 27.9 bits, see alignment E=2e-10 PF00977: His_biosynth" amino acids 219 to 283 (65 residues), 24.2 bits, see alignment E=3.2e-09

Best Hits

Swiss-Prot: 49% identical to PYRDB_GEOUR: Dihydroorotate dehydrogenase B (NAD(+)), catalytic subunit (pyrD) from Geobacter uraniireducens (strain Rf4)

KEGG orthology group: K00226, dihydroorotate dehydrogenase (fumarate) [EC: 1.3.98.1] (inferred from 77% identity to bgf:BC1003_5003)

MetaCyc: 45% identical to dihydroorotate dehydrogenase (catalytic subunit) (Bacillus subtilis subtilis 168)
Orotate reductase (NADH). [EC: 1.3.1.14]

Predicted SEED Role

"Dihydroorotate dehydrogenase, catalytic subunit (EC 1.3.3.1)" in subsystem De Novo Pyrimidine Synthesis (EC 1.3.3.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.3.1, 1.3.98.1

Use Curated BLAST to search for 1.3.1.14 or 1.3.3.1 or 1.3.98.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (316 amino acids)

>GFF123 Dihydroorotate dehydrogenase (NAD(+)), catalytic subunit (EC 1.3.1.14) (Variovorax sp. SCN45)
LADLNIRIGELELRNPVMPASGCFAIEYSEALDLNRLGALVVKSVSPVSRAGNPTPRVAE
AHAGMLNSIGIPSKGLDYYRREVLPAYTGFDTPVVVSISADTVDEFAEAVAEMSLPEVAV
IEANISCPNLEADGMAFAMTPETTYKVVSAIRRKTRHPFWAKLTPNASHIAAVAKAAEEA
GADALVMGNTVLGMAIDVRTRKPRLGNVMGGLSGPAIKPIALRMVHQCYRSVRIPVIGCG
GICSADDAVEFMLAGASAVQVGTASFIDPGVMQKIIDGLEAYCREMGVAHVRDLTGQVKL
DRQLSDRWLRFAQQSG