Protein Info for GFF1211 in Sphingobium sp. HT1-2

Annotation: Imidazolonepropionase (EC 3.5.2.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 401 TIGR01224: imidazolonepropionase" amino acids 26 to 400 (375 residues), 481.3 bits, see alignment E=9e-149 PF01979: Amidohydro_1" amino acids 61 to 378 (318 residues), 70.4 bits, see alignment E=1.7e-23 PF07969: Amidohydro_3" amino acids 112 to 379 (268 residues), 65.5 bits, see alignment E=6.9e-22

Best Hits

Swiss-Prot: 82% identical to HUTI_SPHWW: Imidazolonepropionase (hutI) from Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)

KEGG orthology group: K01468, imidazolonepropionase [EC: 3.5.2.7] (inferred from 82% identity to swi:Swit_4633)

MetaCyc: 62% identical to imidazolonepropionase (Pseudomonas fluorescens)
Imidazolonepropionase. [EC: 3.5.2.7]

Predicted SEED Role

"Imidazolonepropionase (EC 3.5.2.7)" in subsystem Histidine Degradation (EC 3.5.2.7)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.2.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (401 amino acids)

>GFF1211 Imidazolonepropionase (EC 3.5.2.7) (Sphingobium sp. HT1-2)
MTMICDRLWTNARLATMAGDGIGAVEDGVIAARDGRIVYAGPRADAPAFAPVETIDCAGR
WITPGLIDCHTHLVHGGDRAAEFELRLAGASYEEIARAGGGIVSTMRATRAASEAELVAS
ALPRLGALIAEGATTVEVKSGYGLDPVSELRMLRAARQLGRERPVRIITTFLGAHALPPE
YAGNADAYIAMLCDELLPAVAAEGLADAVDAFCEGIGFSPAQTDRLFTAARALGLPVKLH
AEQLSNLHGAALAASHGALSADHLEYLDEDGIAAMARAGTVATLLPGAYYFVRETKLPPI
DGLRRAGVPIALATDCNPGTSPLTSLLLVMNMGATLFRLTVDECLAGVTRNAARALGLSD
EIGTLEVGKRCDLAIWDIERPAELVYRMGYNPLHARIWSGQ