Protein Info for GFF121 in Methylophilus sp. DMC18

Annotation: Caffeyl-CoA reductase-Etf complex subunit CarE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 363 PF01012: ETF" amino acids 28 to 206 (179 residues), 152.6 bits, see alignment E=1.1e-48 PF00766: ETF_alpha" amino acids 235 to 312 (78 residues), 124.8 bits, see alignment E=1.1e-40

Best Hits

Swiss-Prot: 71% identical to FIXB_AZOVI: Protein FixB (fixB) from Azotobacter vinelandii

KEGG orthology group: K03522, electron transfer flavoprotein alpha subunit (inferred from 78% identity to hse:Hsero_2838)

MetaCyc: 71% identical to quinone reductase (NADH,flavodoxin) complex electron transfer flavoprotein component beta subunit (Azotobacter vinelandii)

Predicted SEED Role

"Electron transfer flavoprotein, alpha subunit" in subsystem Acetyl-CoA fermentation to Butyrate

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (363 amino acids)

>GFF121 Caffeyl-CoA reductase-Etf complex subunit CarE (Methylophilus sp. DMC18)
MSEETKKPAPAGKKKFELDERLKAYKGVWVFIEHERGAIHPVSLELLGEGRKLADMLGVE
LAGVVMGPPGETTRSFCQEAFYHGADLCYLIEDPLLQDYRNEPYTKGLTDLVNKYQPEIL
FLGATTLGRDLAGSVATTLKTGLTADCTELNIDLDVRSLLASRPTFGGSLLCTIQTLNYR
PQMATVRPRVMAMPERDATRNGRIIEDTLSMIEDSIITKVLEFIPDEMQDKPQLPFADII
VAGGRGMKKPENFKLIWELAQVLGAEVGATRPVVQAGWVELDRQVGQSGKTVRPKLYIAV
GISGAIQHRVGMDASDVIIAINNDPNAPIFDFAHYGIVGNAMTILPALTEAFKAHLATAR
KAG