Protein Info for GFF1206 in Variovorax sp. SCN45

Annotation: Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01077: NIR_SIR
  • amino acids 142 to 282 (141 residues), 140.1 bits, E=4.4e-45
  • amino acids 442 to 584 (143 residues), 48.5 bits, E=6.9e-17
• PF03460: NIR_SIR_ferr
  • amino acids 374 to 428 (55 residues), 50.6 bits, 1.3e-17

Best Hits

KEGG orthology group: K00381, sulfite reductase (NADPH) hemoprotein beta-component [EC: 1.8.1.2] (inferred from 96% identity to vpe:Varpa_2617)

Predicted SEED Role

"Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2)" in subsystem Cysteine Biosynthesis (EC 1.8.1.2)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (9/10 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)

KEGG Metabolic Maps

• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.2

Use Curated BLAST to search for 1.8.1.2

Protein Sequence (592 amino acids)

>GFF1206 Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2) (Variovorax sp. SCN45)
MYQYTEFDRQFVHQRAAQFRDQLERWQKGRLHEDEFRPLRLQNGWYVQRYAPMLRVAVPY
GELSSRQIRVLARIAREYDEPEAEVYRKAIETQGLLGSQKLPTHYAHFSTRQNVQFNWIP
LAKSADVMDLLASVDMHGIQTSGNCIRNITSDERAGIAIDEIADPRPYAEIMRQWSTLHP
EFAFLPRKFKIAITGATEDRAATGWHDVGLHIVKNEAGEIGFRVQVGGGMGRTPIVGTVL
REFLPWQQIMNYLEAVIRVYNRYGRRDNIYKARIKILVKAEGQRYIDDVDAEYKQILEHD
GAPHTITQAEFDRVAASFVPPTLATRVLQSAEKTDAELRTHAADDVQFARWLARNVAPHK
NPALRAVTLSFKRLKQAPGDASADQLDTLAELADRFSAGEARVTHDQNVVLPWVHAEDLH
ALWLAARAAGLASANVHLLTDMIACPGGDFCALANARSIPIAEAITERYQDLDELDDLGE
IDLHISGCINSCGHHHSGHIGILGVDKDGKEWYQVTLGGSDGSALSGASQAGKVVGPSFS
AAEVPGVIEAVLTTYRDTRESGENFIDTLRRVGPDPFKAAANGARFKVEEAA