Protein Info for GFF1206 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Benzoate-CoA ligase (EC 6.2.1.25)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02262: benzoate-CoA ligase family
  • amino acids 10 to 519 (510 residues), 779.5 bits, E=7.5e-239
• PF00501: AMP-binding
  • amino acids 26 to 386 (361 residues), 265.4 bits, E=7.5e-83
• PF13193: AMP-binding_C
  • amino acids 436 to 511 (76 residues), 80.9 bits, E=1.1e-26

Best Hits

KEGG orthology group: K04110, benzoate-CoA ligase [EC: 6.2.1.25] (inferred from 74% identity to vap:Vapar_0089)

Predicted SEED Role

"Benzoate-CoA ligase (EC 6.2.1.25)" in subsystem Benzoate transport and degradation cluster (EC 6.2.1.25)

MetaCyc Pathways

• benzoate degradation II (aerobic and anaerobic) (1/1 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (4/9 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (6/17 steps found)
• anaerobic aromatic compound degradation (Thauera aromatica) (6/27 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.25

Use Curated BLAST to search for 6.2.1.25

Protein Sequence (523 amino acids)

>GFF1206 Benzoate-CoA ligase (EC 6.2.1.25) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MDTTVAPPSERFNFARHLLELNSGRAAKTALIDDQGRLSYGELADQVRRFSAALSALGLR
REERVLLLMHDSSDWVVAFLGALHAGVVPVAVNTLLTAQDYAYMMANSRAQAALVSAALL
PTLQTALDNLNAQGGHEVKHVIASRATGALPDGVHHFATLVGSHAPAAQAADTHGEEPAF
WLYSSGSTGAPKGTVHTQANLYWTAELYGKGVLALRENDVVFSAAKLFFAYGLGNALSFP
LTVGASVVLMAERPTPQACFKRFKDQQPTVFYGAPTGYGGMLAAADLPPKSEVALRLCSS
AGEALPQDIGQRWTNHFGVEIIDGIGSTEMLHVFLSNRPGDVRYGTTGKPVPGYEIELRG
EDGRPVADGEIGDLYIKGPSAALMYWNNRGKSRDTFQGGWTKAGDKYTRDADGYCTYAGR
NDDMLKVSGIYVSPFEVEATLVQHPAVLEAAVIGKTDADGLTKTKAFVVLKAGAQATEAE
LQAFVKEKLAPYKYPRFIEFLPELPKTATGKIQRFRLREREAG