Protein Info for GFF1203 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Benzoyl-CoA-dihydrodiol lyase (EC 4.1.2.44)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 70% identical to BOXC_AZOEV: Benzoyl-CoA-dihydrodiol lyase (boxC) from Azoarcus evansii

KEGG orthology group: K01692, enoyl-CoA hydratase [EC: 4.2.1.17] (inferred from 84% identity to vpe:Varpa_0109)

MetaCyc: 70% identical to benzoyl-CoA-dihydrodiol lyase monomer (Aromatoleum evansii)
RXN-11054 [EC: 4.1.2.44]

Predicted SEED Role

"Benzoyl-CoA-dihydrodiol lyase (EC 4.1.2.44)" (EC 4.1.2.44)

MetaCyc Pathways

oleate β-oxidation (29/35 steps found)
phenylacetate degradation I (aerobic) (8/9 steps found)
adipate degradation (5/5 steps found)
superpathway of phenylethylamine degradation (9/11 steps found)
benzoyl-CoA degradation I (aerobic) (6/7 steps found)
2-methyl-branched fatty acid β-oxidation (11/14 steps found)
superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
benzoyl-CoA biosynthesis (3/3 steps found)
valproate β-oxidation (7/9 steps found)
adipate biosynthesis (4/5 steps found)
fatty acid β-oxidation I (generic) (5/7 steps found)
fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
fatty acid β-oxidation IV (unsaturated, even number) (3/5 steps found)
methyl ketone biosynthesis (engineered) (3/6 steps found)
(8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
3-phenylpropanoate degradation (5/10 steps found)
benzoate biosynthesis I (CoA-dependent, β-oxidative) (4/9 steps found)
benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (1/5 steps found)
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
Spodoptera littoralis pheromone biosynthesis (4/22 steps found)
platensimycin biosynthesis (6/26 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.17

Use Curated BLAST to search for 4.1.2.44 or 4.2.1.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (555 amino acids)

>GFF1203 Benzoyl-CoA-dihydrodiol lyase (EC 4.1.2.44) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MSATATPPAVDYRTDPSQYRHWKLSFDGDVATLAVDINEDAGIRPGYKLKLNSYDLGVDI
ELHDALNRVRFEHPEVRTVVVTSGKDRVFCSGANIFMLGLSSHAWKVNFCKFTNETRNGI
EDSSQYSGLKFVAALNGACAGGGYELALACDEIVLVDDRSSAVSLPEVPLLGVLPGTGGL
TRVTDKRHVRHDLADIFCTTSEGVRGQKAVDWRLVDSIAKPQQFAAHVKERAAALAAKSD
RPAAGKGVALTRVARTDSADGISYQYVNVAIDRAKRNVTLTVKAPTGAQPKDIAGIEAAG
AAWWPLQMARELDDAILCLRTNELDIGTWLLKTEGDAAAVLASDALMLANKDHWLVRETL
GLMRRTFARLDVSSRSLFALIESGSCFAGALAELAFCADRAYMLALPDDADAAPKLQLGE
FNFGFLPMVNEQSRLQRRFYEETAPMEAARGAIGKALDADAALALGLVTAAPDDIDWADE
VRMALEERAAMSPDALTGLEANLRFAQKENMATRIFGRLTAWQNWIFIRPNAVGEKGALK
VYGKGDKAAFDFNRV