Protein Info for GFF1185 in Sphingobium sp. HT1-2

Annotation: Nitrate transporter NasA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 414 transmembrane" amino acids 30 to 51 (22 residues), see Phobius details amino acids 67 to 86 (20 residues), see Phobius details amino acids 97 to 115 (19 residues), see Phobius details amino acids 121 to 142 (22 residues), see Phobius details amino acids 154 to 174 (21 residues), see Phobius details amino acids 180 to 202 (23 residues), see Phobius details amino acids 228 to 252 (25 residues), see Phobius details amino acids 258 to 277 (20 residues), see Phobius details amino acids 289 to 308 (20 residues), see Phobius details amino acids 314 to 336 (23 residues), see Phobius details amino acids 347 to 367 (21 residues), see Phobius details amino acids 379 to 400 (22 residues), see Phobius details PF07690: MFS_1" amino acids 39 to 363 (325 residues), 146.4 bits, see alignment E=5.4e-47

Best Hits

Swiss-Prot: 49% identical to NASA_BACSU: Nitrate transporter (nasA) from Bacillus subtilis (strain 168)

KEGG orthology group: K02575, MFS transporter, NNP family, nitrate/nitrite transporter (inferred from 85% identity to sjp:SJA_C1-28560)

Predicted SEED Role

"Nitrate/nitrite transporter" in subsystem Nitrate and nitrite ammonification

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (414 amino acids)

>GFF1185 Nitrate transporter NasA (Sphingobium sp. HT1-2)
MATAYWDREGEAASATATTSFWKAGHAPTLIAAFLYFDLAFMVWVLLGPLAPMIAKTLAL
TPAEKGLMVATPTLAGALLRVVNGLLVDRIGPKRSGAISQIIVIAGLFLAWLMGVNSFGG
TLALGVILGFAGASFAIALPLASRWYPPEHQGKAMGLAGMGNSGTVLASLFAPMLAKAFG
WNAVLGLACIPLSIVFVAYMIMAKDAPNAPPAKRLVDYFEPLKTADAWWLMAFYAVTFGG
FVGLAASLPIYFTDQFGLTPIIAGYCTAACVFAGSLVRPMGGALADRIGGVKALMIVYVV
AALALAGVSQATTLASALALFVLAMLALGTGNGSVFQLVPQRFQAEIGVMTGLVGMAGGI
GGFYLASSLGLAKQLTGSFAPGFLIFAGLAVLALLGVALVKAKWRANWTSGARI