Protein Info for GFF1177 in Variovorax sp. SCN45

Annotation: Serine protease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 495 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details TIGR02037: peptidase Do" amino acids 56 to 490 (435 residues), 485.6 bits, see alignment E=7.4e-150 PF00089: Trypsin" amino acids 124 to 279 (156 residues), 65.2 bits, see alignment E=2.4e-21 PF13365: Trypsin_2" amino acids 125 to 258 (134 residues), 127.5 bits, see alignment E=2.1e-40 PF00595: PDZ" amino acids 293 to 375 (83 residues), 46.5 bits, see alignment E=1.2e-15 amino acids 406 to 482 (77 residues), 28 bits, see alignment E=6.9e-10 PF13180: PDZ_2" amino acids 297 to 388 (92 residues), 62.1 bits, see alignment E=1.5e-20 PF17820: PDZ_6" amino acids 324 to 377 (54 residues), 47 bits, see alignment 5e-16 amino acids 436 to 483 (48 residues), 45.3 bits, see alignment 1.7e-15

Best Hits

Swiss-Prot: 47% identical to DEGPL_PSEPG: Probable periplasmic serine endoprotease DegP-like (PputGB1_4377) from Pseudomonas putida (strain GB-1)

KEGG orthology group: K01362, [EC: 3.4.21.-] (inferred from 93% identity to vpe:Varpa_2570)

Predicted SEED Role

"Outer membrane stress sensor protease DegS" in subsystem Proteolysis in bacteria, ATP-dependent

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (495 amino acids)

>GFF1177 Serine protease (Variovorax sp. SCN45)
MNSRLTSPRALVIALATAGVIGAVGAGAYTSARAVGAPATTNAVATAPAAMVTLPDFSTI
TSRDGPAVVNISVTGTMKASDDEAAAEIQGIDPDDPMFQFFRRFQGQMNPRGQQQQRDVP
VRAQGSGFIVDPSGIIITNAHVVKDAKEVTVKLTDRREFRAKVLGADAKTDIAVLKIDAK
NLPVLALGNTKDLKVGEWVLAIGSPFGFENTVTAGVVSAKGRSLPDDSYVPFIQTDVAVN
PGNSGGPLLNTRGEVVGINSQIYSRSGGYQGLSFAIPIDVAIQVKDQIVATGKATHGRLG
VAVQEVNQAFADSFKLDKPEGALVSNIEKGGPGDKAGLKAGDVIRKVNGQPIISSGDLPA
VIGQQAPGKQVTLEVWRQGERQELQAKLGDASDKPAKVAKADADVGQGKLGLALRPLQPQ
EKREASVDNGLLIEDAAGPSALAGVQAGDVLLAINGTPAQSVEQVREVVAKATNKSVALL
IQRGEDKIFVPVRIG