Protein Info for GFF1176 in Methylophilus sp. DMC18

Annotation: Ribonuclease E

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 907 TIGR00757: ribonuclease, Rne/Rng family" amino acids 13 to 423 (411 residues), 532.8 bits, see alignment E=3.1e-164 PF00575: S1" amino acids 37 to 116 (80 residues), 37.8 bits, see alignment E=3.1e-13 PF10150: RNase_E_G" amino acids 120 to 389 (270 residues), 333 bits, see alignment E=2.2e-103 PF20833: RNase_E_G_Thio" amino acids 401 to 485 (85 residues), 107.7 bits, see alignment E=3.9e-35

Best Hits

Predicted SEED Role

"Ribonuclease E (EC 3.1.26.12)" in subsystem RNA processing and degradation, bacterial or Ribosome biogenesis bacterial (EC 3.1.26.12)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.1.26.12

Use Curated BLAST to search for 3.1.26.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (907 amino acids)

>GFF1176 Ribonuclease E (Methylophilus sp. DMC18)
MKRMLFNATQSEELRVAIVDGQKLIDLDIEHAGKEQRKSNIYKGVITRIEPSLEAAFVDY
GTDRHGFLPFKEIARSYFQEKPDQGRARIQDVIKEGQEVIVQVDKDERGSKGAALTTFIS
LAGRYLVLMPNNPRGGGVSRRVEGEDRNELRDTMAQLEVPNGMSIIARTAGIGRNAEELQ
WDLNYLTQLWQAIEDASNFQPGAYLIYQESSLVIRAIRDYFSADIGEILIDTADVHEQAL
QFMNHVMPGNVTRVKLYQDEIPLFSRFQIEHQIESAFAREVRLPSGGAIVIDHTEALVSI
DVNSGRSIKGADIEQTAFNTNLEAAEEVARQMRLRDLGGLVVIDFIDMENQRNQRDVENA
LRDALHHDRARVQMGKISRFGLLEMSRQRLRPSLGETNHMTCPRCNGTGHIRGIESTALH
ILRITQEEAMKDNSAIIQVQLPVEAATFLLNEKRADIHKIEQRTGVEVILIPNIHMETPN
YNILRIRHDDVNEETTQASYKMVDLPSESDYQHRLEQAAAPAKAEALVKGITPATSAPIV
EEKPAPAPVQVTEQKKSFFGVIKSWLGLDEKEVVPSEPEKPARESKEHGRDRNKNRNRRD
RQRDRGQERNERPANERAGKEQQETQHGSKPRQERPAQEKPAQRERQERQANQEVKAETN
QPALTAQSGDNKQERGERGNRRNRHGRRDRRNRDEAPNQNVVESENSVVTETAASVNLAA
APVVADTVPTFAVTETTAAVSPVEVVAAAPIVENPAATVETPVEDTAIQSADDNQTEAPK
AQKPARNRSRAGKPAGKAKAKQADQAVDTSGIELVETAATSSPVVTEATEAPAEAKKKPA
RKRKPSAAKTTETQADSGIQLVETKAEVVNTGVEATQKTKKPRNAAKWKKDEASTGEDGL
VMVETQK