Protein Info for GFF1168 in Xanthobacter sp. DMC5

Annotation: Alanine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 80% identical to SYA_AZOC5: Alanine--tRNA ligase (alaS) from Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)

KEGG orthology group: K01872, alanyl-tRNA synthetase [EC: 6.1.1.7] (inferred from 80% identity to azc:AZC_2854)

Predicted SEED Role

"Alanyl-tRNA synthetase (EC 6.1.1.7)" (EC 6.1.1.7)

MetaCyc Pathways

tRNA charging (19/21 steps found)
superpathway of chorismate metabolism (41/59 steps found)
shinorine biosynthesis (3/8 steps found)
vanchrobactin biosynthesis (2/8 steps found)
enterobactin biosynthesis (4/11 steps found)
ergotamine biosynthesis (4/12 steps found)
(2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
bacillibactin biosynthesis (3/12 steps found)
superpathway of ergotamine biosynthesis (5/18 steps found)
sulfazecin biosynthesis (1/16 steps found)
phosalacine biosynthesis (5/25 steps found)
phosphinothricin tripeptide biosynthesis (5/25 steps found)
corallopyronin A biosynthesis (2/30 steps found)
colibactin biosynthesis (6/38 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (883 amino acids)

>GFF1168 Alanine--tRNA ligase (Xanthobacter sp. DMC5)
MSGVNEIRSSFIDYFVKEGHEAVASSPLVPHNDPTLMFTNAGMVQFKNVFTGVEKRPYSR
AVTSQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFKDRAIELAWNLVTRTFEL
PKDRLLVTVYSEDEEAYGLWKKIAGLSDDRIIRIASSDNFWSMGDTGPCGPCSEIFFDHG
PHIFGGPPGSADQDGDRFIEIWNLVFMQYEQLPGGERVGLPRPSIDTGMGLERISAVLQG
THDNYETDLMRALIAEVAEFTGVDPEGPQKASHRVIADHLRASVFLTADGVLPSNEGRGY
VLRRIMRRAMRHAQLLGAKDPLMHRLVPILVREMGRAYPEIVRAEPLAEETLLLEETRFR
RTLERGLSILEEESQGLVSGASFPGEVAFKLYDTYGFPLDLTQDALRARGISVDTDAFDA
AMARQKAEARANWAGSGEAATDTLWFAVRDKVGATDFLGYDTEKAEGVVTALVRDGAEVA
GLNAGETGLVVLNQTPFYAESGGQVGDTGRMTGEGGIVVEVRATQKKLGDLFLHDVKVES
GTLKTGTALVLEVEHDRRAAVRANHSATHLLHEALRRVLGEHVAQKGSLVAPDRLRFDFS
HPKPLSEAEVAEVEEIANRFVLRNEPVETRILAVDDAIKSGARALFGEKYGEEVRVVSMG
TDDGNGAPYSVELCGGTHVKRTGDIGLISVVSEGAVASGVRRIEALTARAARKHLNGASA
ALIATASTLKAPVAEVEARVAALVEERRKLERELAETRKKLAMGGNQGSGDAVRTVGDIK
LLARQVEGIEIKDLKGLVDEGKKQIGSGVVAIVGVTSDGKAGIVVGVTADLTARVDAVAL
VRRGAEALGGKGGGGRPDMAQAGGPDGDKADAALSAIEAALAG