Protein Info for GFF116 in Xanthobacter sp. DMC5

Annotation: Multidrug transporter EmrE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 114 transmembrane" amino acids 7 to 25 (19 residues), see Phobius details amino acids 34 to 55 (22 residues), see Phobius details amino acids 62 to 83 (22 residues), see Phobius details amino acids 89 to 108 (20 residues), see Phobius details PF00893: Multi_Drug_Res" amino acids 6 to 98 (93 residues), 108.9 bits, see alignment E=7.7e-36

Best Hits

Swiss-Prot: 64% identical to QACE_ECOLX: Quaternary ammonium compound-resistance protein QacE (qacE) from Escherichia coli

KEGG orthology group: K03297, small multidrug resistance protein, SMR family (inferred from 83% identity to xau:Xaut_3102)

MetaCyc: 57% identical to DLP12 prophage; multidrug/betaine/choline efflux transporter EmrE (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-344; TRANS-RXN0-493; TRANS-RXN0-532; TRANS-RXN0-533; TRANS-RXN0-628

Predicted SEED Role

"Ethidium bromide-methyl viologen resistance protein EmrE"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (114 amino acids)

>GFF116 Multidrug transporter EmrE (Xanthobacter sp. DMC5)
MGADHMSYVYLLIAIVAEVIATSALKSAEGFTKLGPSVIVVIGYAIAFFCLSLTLRTLPV
GIAYAIWSGVGIVLVTGFAWIYYGQKLDLAALIGLGLIIAGVVVINLLSKSVAH