Protein Info for GFF1150 in Variovorax sp. SCN45
Annotation: NAD synthetase (EC 6.3.1.5) / Glutamine amidotransferase chain of NAD synthetase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01950, NAD+ synthase (glutamine-hydrolysing) [EC: 6.3.5.1] (inferred from 94% identity to vpe:Varpa_2543)Predicted SEED Role
"NAD synthetase (EC 6.3.1.5) / Glutamine amidotransferase chain of NAD synthetase" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 6.3.1.5)
MetaCyc Pathways
- aspartate superpathway (24/25 steps found)
- NAD de novo biosynthesis I (6/6 steps found)
- NAD de novo biosynthesis II (from tryptophan) (8/9 steps found)
- NAD salvage pathway II (PNC IV cycle) (5/5 steps found)
- NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde (4/4 steps found)
- NAD salvage pathway I (PNC VI cycle) (6/7 steps found)
- superpathway of NAD biosynthesis in eukaryotes (11/14 steps found)
- NAD de novo biosynthesis III (5/6 steps found)
- NAD de novo biosynthesis IV (anaerobic) (5/6 steps found)
- NAD salvage pathway V (PNC V cycle) (4/5 steps found)
- NAD salvage (plants) (6/11 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.3.1.5
Use Curated BLAST to search for 6.3.1.5 or 6.3.5.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (565 amino acids)
>GFF1150 NAD synthetase (EC 6.3.1.5) / Glutamine amidotransferase chain of NAD synthetase (Variovorax sp. SCN45) MTLKLAIAQLNFVVGDLAGNAKKIVDAAREAYSQGARLLLTPELSIAGYAAEDLFLRPAF TEACDDAVKGIAAALADLKDMVVVVGHPTGGSLRSRSVAVQLRHNAASVIKEGRILETYA KRELPNYQVFDERRYFTPGQGTCVFEAGGVSVGLLICEDAWFDQPAELARESGAEVLAVI NASPYHVGKEGERVARMAERARAVGLPLVYAHLVGGQDEVVFDGASFALQADGTTVMQAE SFREKLVFAQLERAPQGGVGFVAEAGAIAAPRDAEAQLWDALVLGVRDYIGKNGFPGAIL GLSGGIDSALVLAIAVDALGKDKVRAVMMPSPYTADISWIDARDMAGRLGVRYDEISIRH TFESFKGALAEEFKGRPEDTAEENIQARIRGTLLMGLSNKFGSIVLTTGNKSEMATGYCT LYGDMAGGFAVIKDLLKTTVFALARWRNAHDPYGMGAEPIPERIITRPPSAELRPDQTDQ DSLPPYDILDGILARYMQDDEGIDEIIAAGYERAVVERVARLIRINEYKRRQAPVGIRVT HRSFGKDWRYPITSKFNETAGAQKP