Protein Info for GFF1140 in Pseudomonas sp. DMC3

Annotation: Aerobic C4-dicarboxylate transport protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 435 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details amino acids 50 to 68 (19 residues), see Phobius details amino acids 80 to 101 (22 residues), see Phobius details amino acids 146 to 169 (24 residues), see Phobius details amino acids 189 to 212 (24 residues), see Phobius details amino acids 223 to 246 (24 residues), see Phobius details amino acids 265 to 282 (18 residues), see Phobius details amino acids 302 to 322 (21 residues), see Phobius details amino acids 334 to 379 (46 residues), see Phobius details PF00375: SDF" amino acids 12 to 405 (394 residues), 352 bits, see alignment E=2.2e-109

Best Hits

Swiss-Prot: 58% identical to DCTA_CHRVO: C4-dicarboxylate transport protein (dctA) from Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)

KEGG orthology group: None (inferred from 95% identity to pfo:Pfl01_2773)

MetaCyc: 56% identical to C4 dicarboxylate/orotate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-121; TRANS-RXN-121A; TRANS-RXN-121C; TRANS-RXN-122A; TRANS-RXN0-451; TRANS-RXN0-517; TRANS-RXN0-553

Predicted SEED Role

"Aerobic C4-dicarboxylate transporter for fumarate, L-malate, D-malate, succunate" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (435 amino acids)

>GFF1140 Aerobic C4-dicarboxylate transport protein (Pseudomonas sp. DMC3)
VETSKSRWYSQLYVQVLIGIVIGAAIGYFVPDIGAKLQPFADGFIKLIKMLLAPIIFGTV
VVGIAKMGSIKEVGRIGVKALIYFEILSTIALVVGLIVVNVVKPGAGMNINAGTLDGSAV
NKYSQAASEQGGLVEFFLNIIPHTFLGAFSNGVMLQVILLSVLMGVALVQMGETSKPLIN
TIDLFLQGLFKIVAMVMRLAPLGAGAGMAFTIGKYGIGTLLSLGQLLVALYITTLIFIVV
VLGAVARWSGMPLMQFLRYFKDEILITLGTCSTEAVLPRMMVKLEKLGCKKSVVGMVLPT
GYTFNADGTCIYLTMAAIFIAQATNTPLTFMDQMILLGVFLLTSKGSAGVAGAGFVTLAA
TLTTIHSIPLVGLVLLLGIDRFLNEARAVTNLIGNGIGTIAIAKWDNSFDVEACEREIAA
MKHAKAERKSLLAHK