Protein Info for GFF114 in Pseudomonas sp. DMC3

Annotation: Protein FixB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 406 PF01012: ETF" amino acids 98 to 210 (113 residues), 52.4 bits, see alignment E=5.8e-18 PF00766: ETF_alpha" amino acids 279 to 354 (76 residues), 86.6 bits, see alignment E=9.1e-29

Best Hits

KEGG orthology group: K03522, electron transfer flavoprotein alpha subunit (inferred from 97% identity to pfo:Pfl01_5221)

Predicted SEED Role

"Electron transfer flavoprotein, alpha subunit" in subsystem Acetyl-CoA fermentation to Butyrate

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (406 amino acids)

>GFF114 Protein FixB (Pseudomonas sp. DMC3)
MSDIIRRDPRAEWIARNRLHPLHAAMQPAQHSWMGPNGVIRKNLHGMGFIGPNGIKRIDR
SGAQQGGAVKRSAAVEVQLPLHQVSAPAFYISVVPDMVGGRLSSHDRDLLGLAHQLAGSD
GAVLAVVFGEHKESAFETAGVDRLLVLEGEEFSGYAPEQRVLGLRAVDNQFNPRHWLLPD
SRSGGGELGRRFAAALGERPATRVWQVKDQECIGRAGAGLQDLARPVARLILAAAECAEP
VSETRHEALPVELSTSVARSLSRIEDLGAVAVDPAAIPMAEAEFIFSGGNGVKDWQLFHE
TAAALGATEGASRVAVDDGFMARDRQVGASGTWVTARVYVAVGISGAIQHLQGIGACDKV
VAINLDPGCDMIKRADLSVIGESAEILQALIEAVAAYRNEAKRDAA