Protein Info for GFF1133 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Sensor kinase CitA, DpiB (EC 2.7.3.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 552 transmembrane" amino acids 32 to 51 (20 residues), see Phobius details amino acids 189 to 209 (21 residues), see Phobius details PF17203: sCache_3_2" amino acids 56 to 185 (130 residues), 75 bits, see alignment E=1e-24 PF14501: HATPase_c_5" amino acids 442 to 537 (96 residues), 42.4 bits, see alignment E=8.8e-15 PF02518: HATPase_c" amino acids 446 to 549 (104 residues), 64.3 bits, see alignment E=2e-21

Best Hits

Swiss-Prot: 84% identical to CITA_KLEPN: Sensor histidine kinase CitA (citA) from Klebsiella pneumoniae

KEGG orthology group: K07700, two-component system, CitB family, cit operon sensor histidine kinase CitA [EC: 2.7.13.3] (inferred from 100% identity to sec:SC0047)

MetaCyc: 42% identical to sensor histidine kinase DpiB (Escherichia coli K-12 substr. MG1655)
Histidine kinase. [EC: 2.7.13.3]

Predicted SEED Role

"Sensor kinase CitA, DpiB (EC 2.7.3.-)" in subsystem Orphan regulatory proteins (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (552 amino acids)

>GFF1133 Sensor kinase CitA, DpiB (EC 2.7.3.-) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MQENTVIKYLNTNMFIREITRWFARHSFQNRIFLLILFTSTIVMLAITWYLTDITKERLH
YQVGQRALIQAMQISAMPELVEAVEAHDLSRIKALIDPMRSFSDATYITVGNEKGQRLYH
VNPDEIGKYMEGGDSDDALYNAKSYVSVRKGSLGSSLRGKSPIQDSTGKVIGIVSVGYTL
EQLESWLNLQISSLLFPMALLLLVLLYCARRFSLHIKKQMLNMEPQQLSQLLIQQSVLFE
SVFEGLIAIDSHHRITAINQTARRLLNLSQPESELIGYAIHHVVLPEAFFYDAPQKNKKD
EIVTFNQMKVIASRMAVIIDNQPQGWVISFRNKDDINTLSLQLSQVRQYADNLRAVQHEH
RNLISTIAGLLFLKRYDNALALIQQQSESHQKVLDFISHNFRDHHLAGLLIGKYYRAKEL
GLELIFDPSSFVEHLPSSLSHNEWISIVGNLLDNAYNATLLNREGSRQIECLINSEGNEV
IIEVADQGCGIDENIRQHIFERGVTTHNNGDHGIGLWLVHSYVKQANGDIIVDNNNPFGT
IFTLYIPFTREE